生物加工过程Issue(5)：31-36,6.DOI:10.3969/j.issn.1672-3678.2015.05.006
耐高渗拟布氏乳杆菌的富集选育
Breeding for high osmosis tolerance of Lactobacillus parabuchneri
冯晨龙 1葛人杰 1段俐 1王丽丽1
作者信息
- 1. 河北科技大学 生物科学与工程学院，河北 石家庄 050018
- 折叠
摘要
Abstract
Lactobacillus parabuchneri K1 can effectively convert fructose to mannitol under anaerobic conditions. However, as fructose concentration increases, cell growth and the yield of mannitol decrease. A directed mutation experiment was designed to improve the cell growth rate and the conversion of L�parabuchneri K1 at high fructose concentration. A 55⁃day continuous subculture was conducted using a 10⁃L fermenter with four⁃time gradient increasing fructose concentration from 270 to ultimate 300 g/L. L�parabuchneri K2, a mutant resistant to high osmotic pressure, increased fructose consumption by 103% in a 2⁃L fermenter at 300 g/L fructose. Fermentation period was shortened by 46�4% comparing with the K1 strain�The mannitol yield was reached to 189 g/L, 94�0% of theoretical conversion.关键词
拟布氏乳杆菌/定向筛选/甘露醇Key words
Lactobacillus parabuchneri/directional screening/mannitol分类
化学化工引用本文复制引用
冯晨龙,葛人杰,段俐,王丽丽..耐高渗拟布氏乳杆菌的富集选育[J].生物加工过程,2015,(5):31-36,6.
