生物加工过程Issue(5)：74-78,5.DOI:10.3969/j.issn.1672-3678.2015.05.014
阿魏菇与胶红酵母共培养产漆酶对活性艳蓝W RV的脱色
Decolorization ofreactive brilliant blue dye with laccase produced by the co-culture of Pleurotus ferulae and Rhodotorula mucilaginosa
王寒 1彭林 2丁重阳 1顾正华 1张梁 2石贵阳1
作者信息
- 1. 江南大学 生物工程学院 工业生物技术教育部重点实验室，江苏 无锡 214122
- 2. 江南大学 粮食发酵工艺与技术国家工程实验室，江苏 无锡 214122
- 折叠
摘要
Abstract
The decolorization of the reactive brilliant blue W⁃RV by laccase from the co⁃culture of Pleurotus ferulae and Rhodotorula mucilaginosa was studied under different reaction conditions,including pH,temperature,dye concentration and amount of laccase.Results indicated that efficient decolorization of reactive brilliant blue W⁃RV was obtained at acidic pH and high temperature was unfavourable. The decolorizaiton of reactive brilliant blue W⁃RV achieved a stable level after a 7⁃h reaction in different dye concentrations. The decolorization rate increased with the laccase amount until 200 U/L and the excess laccase (more than 200 U/L) did not improve the decolorization.The optimized reaction conditions were pH 4.5,30 ℃,100 mg/L reactive brilliant blue W⁃RV and 200 U/L laccase.Under these conditions,the highest decolorization by laccase was 89.91%.关键词
漆酶/阿魏菇/胶红酵母/活性艳蓝WRV/脱色Key words
laccase/Pleurotus ferulae/Rhodotorula mucilaginosa/reactive brilliant blue W-RV/decolorization分类
资源环境引用本文复制引用
王寒,彭林,丁重阳,顾正华,张梁,石贵阳..阿魏菇与胶红酵母共培养产漆酶对活性艳蓝W RV的脱色[J].生物加工过程,2015,(5):74-78,5.
