现代检验医学杂志Issue(4)：10-13,4.DOI:10.3969/j.issn.1671-7414.2015.04.003
人羧基肽酶 H不同区段抗原的克隆表达及其初步应用
Cloning,Expression and Diagnostic Application of Different Fragments of Human Carboxypeptidase
摘要
Abstract
Objective To obtain different fragments of human carboxypeptidase H,and evaluate the diagnostic application of the recombination carboxypeptidase H in detecting autoantibody.Methods The coding gene of carboxypeptidase H was ob-tained by RT-PCR.The corresponding prokaryotic expression vectors were constructed and transformed into E.coli to in-duce the expression of the recombination different fragments of carboxypeptidase H.Using these antigen fragments as the coating antigens,the enzyme-linked immunosorbent assay (ELISA)was established for the detection of carboxypeptidase H autoantibody in 95 newly diagnosed type 2 diabetes patients.Results Three fragments of human carboxypeptidase H were obtained,in which the 42~476aa fragment antigen was ideal one.Using the full-length carboxypeptidase H as coating anti-gen,the positive rate of carboxypeptidase H autoantibody was 8.42%.Conclusion Because of the favorable antigenicity,the 42~476aa fragment antigen of carboxypeptidase H could be the candidate antigen for discrimination and diagnosis of latent autoimmune diabetes in adults.关键词
人羧基肽酶H/自身抗体/新诊断2型糖尿病/成人隐匿性自身免疫性糖尿病Key words
human carboxypeptidase H/autoantibody/newly diagnosed type 2 diabetes/latent autoimmune diabetes in adults分类
医药卫生引用本文复制引用
胡纪文,冯晓燕,张贺秋,杨锡琴,宗克亮,郭兰芹,宋晓国,王国华,刘喜明,朱翠侠,赵琰枫..人羧基肽酶 H不同区段抗原的克隆表达及其初步应用[J].现代检验医学杂志,2015,(4):10-13,4.基金项目
十二五国家高技术研究发展计划（863计划）课题，编号2011AA02A113；深圳市伯劳特生物制品有限公司委托课题。 （）
