苏州科技学院学报（自然科学版）Issue(3)：36-42,7.
基于T-RFLP技术的拙政园土壤微生物多样性分析
T-RFLP analysis of soil microbial diversity in Humble Administrator's Garden
吴琼 1陈紫丹 1邱业先1
作者信息
- 1. 苏州科技学院 化学生物与材料工程学院，江苏 苏州 215009
- 折叠
摘要
Abstract
Terminal restriction fragment length polymorphism (T-RFLP) technique was used to analyze microbial diversity in 3 points of sediment and 3 points of soil in Humble Administrator's Garden. The results show that a-mong the sediment bacteria ,T-RFs fragments with the sizes of 181 bp ,211 bp were the dominant bacteria. A-mong soil bacteria ,T-RFs fragments with the sizes of 91 bp ,205 bp were the dominant bacteria. Among sediment fungi, species of each site were not identical. Among soil fungi ,T-RFs fragment with the size of 355 bp was the dominant fungus. Bacterial and fungal diversity of sediment and soil in Humble Administrator's Garden were dif-ferent. Bacterial diversity was significantly higher than that of fungi. This study has provided valuable data for microbial diversity and community structure of Suzhou gardens.关键词
拙政园/微生物多样性/末端限制性片段长度多态性/底泥/土壤Key words
Humble Administrator's Garden/microbial diversity/T-RFLP/sediment/soil分类
生物科学引用本文复制引用
吴琼,陈紫丹,邱业先..基于T-RFLP技术的拙政园土壤微生物多样性分析[J].苏州科技学院学报（自然科学版）,2015,(3):36-42,7.
