心血管外科杂志（电子版）Issue(2)：81-85,5.
间断灌注保存对离体猪心冠状动脉保护效果的观察
Analysis of the protective effect with intermittent perfusion on coronary artery in isolated pig heart
摘要
Abstract
Objective To compare the protective effect of different perservation on coronary artery in isolated heart of pigs.Methods A total of 36 Bama-miniature-pigs′donor hearts were arrested with Celsior cardioplegia solution enhanced with Diazoxide ( DE,30 μmol /L).Isolated hearts were stored in this solution in immersion storage group or place in a device that providing intermittent perfusion with 50,100,150,200,250 ml solution respectively every 2 hours in the intermittent perfusion group at 4 ℃ for 10 hours (n =6 per group).After storage,left anterior descending coronary artery samples were frozen ,extracted,and analyzed the endothelial nitric oxide synthase (eNOS),endothelin-1 (ET-1),intercellular adhesion molecule -1 (ICAM-1),von Willebrand factor (vWF) and ultrastructure by immunohistochemisty and RT -PCR.Results The expression of eNOS protein and mRNA in the immersion preservation group was minimum ,in perfusion preservation group,eNOS protein and mRNA increased with the increasing of capacity ; the expression of ET-1,ICAM-1,vWF protein and mRNA were highest in immersion preservation,in perfusion preservation group,they increased with the reducing of capacity ; in 200 ml and 250 ml perfusion group,there was no significant difference in indexes except ICAM -1 protein.Both group retained relatively complete coverage better endothelial cells ultrastructure and fewer vacuoles and subendothelial layer was thicker.Conclusion These results indicate that the intermittent perfusion is optimal means on coronary artery in isolated heart preservation with the support of Celsior cardioplegia solution enhanced with DE (30 μmol /L).They can better protect the coronary vasomotor factors , reduce vascular inflammatory mediators released and protect coronary artery endothelial cells′structure.And it is more superior protection when the heart is perfused 200 ml solution every 2 hours.关键词
冠状血管/间断灌注/血管舒缩因子/Clesior液Key words
Coronary vessels/Intermittent perfusion/Endothelium-derived factor/Celsior solution分类
医药卫生引用本文复制引用
胡志斌,严志焜,王海涛,孟群,潘晓华..间断灌注保存对离体猪心冠状动脉保护效果的观察[J].心血管外科杂志（电子版）,2015,(2):81-85,5.基金项目
浙江省自然科学基金 （）
