中国动物检疫2015，Vol.32Issue(9)：64-68,5.
牛分枝杆菌蛋白基因相互作用研究工具及其进展
M. bovis Protein Interaction and Its Investigative Tools
摘要
Abstract
Several tools were applied in the M.bovis protein interaction investigation. First,the yeast two-hybrid was ap-plied to verify two protein interactions in-vivo. Second,mutant strains were constructed to elucidate the mechanism of protein function. The strategies include:(1) amplification of knockout DNA;(2)preparation ofMycobacterium/pJV53 competent cells;(3)transportation of knockout DNA to competent cells;(4)screening and identification of positive colonies. The third,GST pull-down was applied to investigate known protein and target protein interaction. RD1 was in-vestigated intensively inM. bovis protein interactions. The mechanisms were found such as that dimmer was formed by ESAT-6 and CFP10,interaction was existed in Rv3873,Rv3866, Rv3868 and CFP-10,and ESAT-6 achieved its function only after it was combined with CFP-10. These findings suggest that elucidation of the interactions could facilitate the development of efficient diagnostic reagents and vaccines for bovine tuberculosis.关键词
牛结核分枝杆菌/蛋白/相互作用Key words
M. bovis/protein/interaction分类
农业科技引用本文复制引用
张喜悦,史扬,范伟兴..牛分枝杆菌蛋白基因相互作用研究工具及其进展[J].中国动物检疫,2015,32(9):64-68,5.基金项目
科技基础性工作专项(2012FY111000) （2012FY111000）
现代农业(奶牛)产业技术体系建设项目(CARS37) （奶牛）
