中国动物检疫2015，Vol.32Issue(9)：72-76,5.
鸡传染性法氏囊病血清抗体间接ELISA检测方法的建立
Development of an Indirect ELISA for the Detection of Antibodies against Infectious Bursal Disease Virus in Chickens
张华 1南文龙 2巩明霞 1张悦勇 1彭大新 1陈义平3
作者信息
- 1. 中国动物卫生与流行病学中心,山东青岛 266032
- 2. 扬州大学兽医学院,农业部畜禽传染病重点开放实验室,江苏扬州 225009
- 3. 青岛农业大学,山东青岛 266109
- 折叠
摘要
Abstract
In order to develop an indirect ELISA for the detection of antibodies against IBDV, VP2 gene(1nt~708nt) was amplified by RT-PCR and expressed using the pET-28a vector inEscherichia coli BL21. The recombinant protein was purified through Ni-chelating affinity chromatography and based on it,an indirect ELISA was developed. To verify the performance of this assay, 156 clinical serum samples were detected by the assay and IDEXX ELISA kit in paral-lel. The results showed that VP2 gene was successfully amplified by RT-PCR and expressed inE.coli BL21 with IPTG induction. Compared to IDEXX ELISA kit, the specificity,sensitivity,and coincidence rate of the developed indirect ELISA were 90.24%,95.65% and 94.23% respectively.The results demonstrated the assay was sensitive and spe-cific,and could be used to detect antibodies against IBDV in chicken sera.关键词
传染性法氏囊病/VP2/原核表达/间接ELISAKey words
IBDV/VP2/prokaryotic expression/indirect ELISA分类
农业科技引用本文复制引用
张华,南文龙,巩明霞,张悦勇,彭大新,陈义平..鸡传染性法氏囊病血清抗体间接ELISA检测方法的建立[J].中国动物检疫,2015,32(9):72-76,5.
