中国康复理论与实践Issue(9)：1012-1015,4.DOI:10.3969/j.issn.1006-9771.2015.09.006
电刺激小脑顶核对脑缺血再灌注后大鼠脑组织损伤的影响及其机制
Effect of Electrical Stimulation to Cerebellar Fastigial Nucleus on Cerebral Ischemia-reperfusion Injury in Rats
何兰英 1罗勇 2王健 3董为伟1
作者信息
- 1. 成都市第二人民医院神经内科，四川成都市610017
- 2. 重庆医科大学附属第一医院神经内科，重庆市400016
- 3. 重庆市神经病学重点实验室，重庆市400016
- 折叠
摘要
Abstract
Objective To investigate the effect of electrical stimulation to cerebellar fastigial nucleus on expression of nuclear fac-tor-kappa B (NF-кB) P50, tumor necrosis factor-α(TNF-α) and Bcl-xL mRNA in rats brain after cerebral ischemia-reperfusion. Methods Sprague-Dawley rats were randomly divided into normal control group (NC group), cerebral ischemia-reperfusion group (I/R group), fasti-gial nucleus stimulation (FNS) group, and fastigial nucleus lesion (FNL) group. A focal cerebral ischemia-reperfusion model was established with middle cerebral artery occlusion (MCAO). 7 and 14 days after operation, the infarct volume was measured, and the protein of NF-кB P50 in rats brain was detected with Western blotting;the expression of TNF-αand Bcl-xL mRNA was detected with RT-PCR. Results Com-pared with I/R group, the expression of NF-кB P50 protein increased in FNS group (P<0.05), with the decrease of expression of TNF-αmRNA (P<0.01) and increase of Bcl-xL mRNA (P<0.05), while the infarct size decreased (P<0.01). There was no significant difference be-tween FNL group and I/R group for all the measurements (P>0.05). Conclusion FNS could induce the expression of P50 protein and Bcl-xL mRNA, and inhibit the expression of TNF-αmRNA, and reduce infarct size, which may associated with the neuroprotection of central ner-vous system from injury.关键词
脑缺血再灌注损伤/电刺激/小脑顶核/核因子-κB/肿瘤坏死因子-α/Bcl-xL/大鼠Key words
cerebral ischemia-reperfusion/electrical stimulation/cerebellar fastigial nucleus/focal factor-kappa B/tumor necrosis fac-tor-α/Bcl-xL/rats分类
医药卫生引用本文复制引用
何兰英,罗勇,王健,董为伟..电刺激小脑顶核对脑缺血再灌注后大鼠脑组织损伤的影响及其机制[J].中国康复理论与实践,2015,(9):1012-1015,4.
