中国水稻科学Issue(5)：457-466,10.DOI:10.3969/j.issn.1001-7216.2015.05.002
一个由可变剪接造成的水稻开颖不育突变体ohms1的鉴定及基因定位
Characterization and Gene Mapping of an Open Hull Male Sterile Mutant ohms1 Caused by Alternative Splicing in Rice
摘要
Abstract
A rice floral organ development mutant was obtained from the 60 Co-γ-treated indica restorer line Zhonghui 8015 and termed asopenhullandmalesterile1 (ohms1).The mutant was characterized as open hull and lemma-and palea-like structure between anthers and stigma that made the spikelet of the mutant showed three“triodia-like”flower glumes.The mutant was self-sterile but 60%-70% pollen were fertile.Genetic analysis and gene mapping showed ohms1 was controlled by a single ressessive gene and the mutant gene was fine mapped to a region of 42 kb on the short arm of chromosome 3 between markers KY2 and KY2 9 .Sequence analysis of the four open reading frames(ORFs)in this region revealed that LOC_Os03g11614 was probably corresponding to ohms1,which encoded a MADS-box gene allelic with OsMADS1 ,and had a single nucleotide transformation (A to G)at the bottom of the fifth intron.Enzyme digestion and cDNA sequencing furtherly indicated that the variable splicing was responsible for the delection of the sixth exon of ohms1 ,but no structure changes in MADS domain or amino acid frameshift occurred.Additionally, real-time fluorescence quantitative PCR analysis showed that the expression level of OHMS1 decreased significantly and the expression level of rice flowering factors and floral glume development-related genes changed significantly.All those results demonstrated that OHMS1 may play an important role in rice floral organ development,particularly in floral glume development and floret primordium differentiation.关键词
水稻/开颖不育/基因定位/可变剪接Key words
rice/open hull male sterile/gene mapping/alternative splicing分类
生物科学引用本文复制引用
孙廉平,吴玮勋,曹立勇,程式华,张迎信,张沛沛,杨正福,占小登,沈希宏,张振华,胡霞,轩丹丹..一个由可变剪接造成的水稻开颖不育突变体ohms1的鉴定及基因定位[J].中国水稻科学,2015,(5):457-466,10.基金项目
国家科技支撑计划资助项目(2011BAD35B02) （2011BAD35B02）
国家转基因重大专项(2011ZX08001-002) （2011ZX08001-002）
浙江省自然科学基金资助项目(LQ14C130003) （LQ14C130003）
中国农业科学院创新工程超级稻育种创新团队、水稻杂种优势研究机理研究创新团队(CAAS-ASTIP-2013-CNRRI) （CAAS-ASTIP-2013-CNRRI）
