中国水稻科学Issue(5)：535-545,11.DOI:10.3969/j.issn.1001-7216.2015.05.011
2013－2014年云南主要稻区病毒病调查及RSV的分子变异
Investigation of Rice Virus Diseases and Analysis of the Molecular Variation of RSV Isolates in the Main Rice-growing Areas of Yunnan Province from 2013 to 2014
摘要
Abstract
Viral disease investigation in main rice planting areas in Yunnan Province from 2013 to 2014 showed that rice stripe disease,southern rice black-streaked dwarf disease,rice dwarf disease and rice ragged stunt disease were the four major viral diseases threaten rice production in Yunnan Province.Among them,rice stripe disease remained widely distributed in Yunnan with the incidence up to 1 5 .0% in some fields followed by rice dwarf disease occurred in some areas with the highest incidence of 10.0%;and the average incidence of southern rice black-streaked dwarf disease was 5 .3%;rice ragged stunt disease occurred with a low incidence.Molecular variation in rice stripe virus (RSV)isolates was analyzed based on the nucleotide sequence of intergenic region (IR)of RNA3 and RNA4.Phylogenetic analysis showed that all of Yunnan RSV isolates could be divided into two distinct clusters.The isolates from Japan,Korea and other provinces of China belonged to groupⅠ,whereas Yunnan isolates fell into both of the two groups and most of them form a distinct Yunnan population of group Ⅱ.The length of IR3 and IR4 varied with RSV isolates,which could be divided into three types of A,B and C according to the difference in the length per 10 nucleotides.Recombination might be occurred during the evolutionary process of RSV since 1 7 to 2 1 nucleotides of IR3 and IR4 of some Yunnan isolates shared some identical sequence with their host plants,rice,wheat or barley.We supposed that the high molecular variation of IR3 and IR4 in Yunnan isolates might result from the special geographical and ecological conditions in Yunnan Province.关键词
水稻病毒病/水稻条纹病毒/基因间隔区/分子变异Key words
rice virus disease/rice stripe virus/intergenic region/molecular variation分类
农业科技引用本文复制引用
张水英,李凡,谭冠林,任国敏,李梅蓉,李月月,兰平秀,桂富荣,王海宁,朱书生..2013－2014年云南主要稻区病毒病调查及RSV的分子变异[J].中国水稻科学,2015,(5):535-545,11.基金项目
云南省教育厅科学研究基金重大专项(ZD2012005) （ZD2012005）
云南省高校科技创新团队支持计划资助项目(云教科[2014]22号).因间隔区(IR3)及 RNA4基因间隔区(IR4)开展了分子变异分析.根据不同 RSV分离物 IR3、IR4核苷酸序列构建系统进化 （云教科[2014]22号）
