作物学报Issue(11)：1640-1647,8.DOI:10.3724/SP.J.1006.2015.01640
小麦粒重基因TaGW2-6A等位变异的组成分析及育种选择
Composition and Selection ofTaGW2-6A Alleles for Wheat Kernel Weight
摘要
Abstract
TaGW2 on chromosome 6A is a key gene governing kernel size of wheat (Triticum aestivumL.). There are a single T-base insertion in the eighth exon ofTaGW2(977 bp) and two SNPs (Hap-6A-A and Hap-6A-G) in the promoter region. In this study, 316 wheat varieties (lines) were detected by high resolution melting curve (HRM) analysis andHap-6A-P1/P2 molecular marker forTaGW2-6A allelic variations and their correlations with kernel length, kernel width, and thousand-kernel weight were analyzed. Furthermore, theTaGW2-6Aallelic variations were traced in the pedigree of the famous large-kernel variety Zhoumai 22. In the 977 bp position, 61 and 255 lines were detected with and without the T-base insertion, which were designated 977T and 977-genotypes, respectively. In the 977T genotypes, 29 lines were Hap-6A-A (TA) haplotype and 32 lines were Hap-6A-G (TG) haplotype. In the 977-genotypes, 160 lines were Hap-6A-A (-A) haplotype and 95 lines were Hap-6A-G (-G) haplotype. Sig-nificant difference was found in kernel length (P < 0.05), kernel width (P < 0.001) and thousand-kernel weight (P < 0.001) be-tween 977T and 977-genotypes. Similarly, significant difference was also found in kernel length (P < 0.05), kernel width (P < 0.05) and thousand-kernel weight (P < 0.001) between Hap-6A-A and Hap-6A-G haplotypes. The allelic variation inTaGW2-6A encoding region and the promoter region jointly contributed to kernel size, and the TA haplotype was superior to-A, TG, and-G haplotypes in increasing kernel width and weight. According to pedigree analysis, the Zhoumai 22 inherited the TA haplotype from the parent Huixianhong, not from the popular parent Zhou 8425B. This haplotype is inheritable stably but tends to be lost in the process of wheat breeding. The results of this study provide not only a high-throughput molecular technique to detect wheat TA haplotype but also breeding materials in marker-assisted selection of wheat.关键词
小麦籽粒性状/TaGW2基因/等位变异/高分辨率熔解曲线(HRM)/分子标记辅助选择Key words
Grain traits of wheat/TaGW2/Allelic variation/High resolution melting curve (HRM)/Marker-assisted selection引用本文复制引用
寇程,高欣,李立群,李扬,王中华,李学军..小麦粒重基因TaGW2-6A等位变异的组成分析及育种选择[J].作物学报,2015,(11):1640-1647,8.基金项目
本研究由国家转基因生物新品种培育科技重大专项(2013ZX08002-003),陕西省科技统筹创新工程计划项目(2014KTZB02-01-01)和陕西省重点科技创新团队计划项目(2014KCT-25)资助。 （2013ZX08002-003）
