川北医学院学报Issue(1)：6-10,5.DOI:10.3969/j.issn.1005-3697.2015.01.02
4个Y-miniSTR基因座荧光标记复合扩增体系的建立
Development of a new quadruplex ChrY miniSTR system
摘要
Abstract
Objective:A quadruplex of primers aimed at 4 Y-chromosomal short tandem repeat ( Y-STR) loci ( DYS456, DYS392,DYS439,Y-GATA-H4) was built. The length of 4 amplicons was all shorter than 150bp which was called miniSTR. An exami-nation was carried out for sensitivity,DNA database compatibility and degraded DNA typing ability of the quardplex. Methods:4 prim-ers of Y-STR(DYS456,DYS392,DYS439,Y-GATA-H4) were specially selected for short amplicons. The front primer of DYS456, DYS392,DYS439,Y-GATA-H4 was labeled with FAM,JOE,ROX,TAMRA separately. DNA samples were collected from forensic ca-ses. And degraded samples were made by ultrasonic impact treatment. Ladder of the quadruplex was amplified from dilution of Y23 Lad-der with the quadruplex primers. The quadruplex was compared to promega Y23 for sensitivity,DNA database compatibility and highly degraded DNA typing ability. Results:Sensitivity of the quardplex was the same as Y23 in both common samples and highly degraded samples. As a result of test on degraded DNA samples using the quadruplex systems,the systems proved to be an more effective tools for analyzing degraded DNAs than Y23. And there were no differences in DNA typing results which means great DNA database compatibili-ty. Conclusion:The quadruplex of Y-miniSTR in addition to the commercial available Y-STR multiplex kits are highly useful for foren-sic practices of degraded DNA samples.关键词
法医物证学/复合扩增体系/miniSTR/Y染色体/降解DNA/低模版DNAKey words
Forensic Genetics/Multiplex kits/MiniSTR/Ychromosome/Degraded DNA/Low temple DNA分类
社会科学引用本文复制引用
王惠,白小刚,夏昱,李庆庆,何望,梁伟波..4个Y-miniSTR基因座荧光标记复合扩增体系的建立[J].川北医学院学报,2015,(1):6-10,5.基金项目
国家自然科学基金项目(81471827,81202387) （81471827,81202387）
四川省公安厅重点攻关及应用创新项目(201303) （201303）
