法医学杂志Issue(1)：7-10,14,5.DOI:10.3969/j.issn.1004-5619.2015.01.002
大鼠脑挫伤后 caspase-3和 HAX-1的表达
Expression of caspase-3 and HAX-1 after Cerebral Contusion in Rat
摘要
Abstract
Objective To observe the expression pattern of caspase-3 and HCLS1-associated protein X-1 (HAX-1) at different time after cerebral contusion in rat, and explore the new method for estimating the injury interval. Methods The cerebral contusion model was established using adult SD male rats. Then the rats were randomly allocated into 8 groups: 2 h, 6 h, 12 h, 1 d, 3 d, and 7 d after cerebral con-tusion, sham-operation and normal control. Expression of caspase-3 and HAX-1 protein after cerebral contusion in rat was detected by Western blotting. Laser scanning confocal microscope was used to ob-serve the number of HAX-1 positive cells and TUNEL-stained cells after cerebral contusion. Results The expression of caspase-3 increased parallelly with the time after cerebral contusion and reached the peak value on 3 d. The expression of caspase-3 decreased gradually and still maintained a high level expression on 7 d (P<0.05). The expression of HAX-1 positive cell went up after injury, and reached the peak value at 6 h (P<0.05), then turned down gradually after 12 h and went out of detection after 3 d. The number of TUNEL-stained cells increased obviously at 2 h and reached the peak value on 3 d. The number of TUNEL-stained apoptotic cells decreased gradually and still maintained a high level expres-sion on 7 d (P<0.05). Conclusion The expression of caspase-3 and HAX-1 after cerebral contusion has time sequential regularity, which may provide new evidence for forensic diagnosis of cerebral contusion interval.关键词
法医病理学/脑损伤/半胱氨酸天冬氨酸蛋白酶 3/造血干细胞特异性相关结合蛋白-1/大鼠Key words
forensic pathology/brain injuries/caspase-3/HCLS1-associated protein X-1/rats分类
社会科学引用本文复制引用
李周儒,滕道辉,董国凯,殷文江,蔡红星..大鼠脑挫伤后 caspase-3和 HAX-1的表达[J].法医学杂志,2015,(1):7-10,14,5.基金项目
江苏省“六大人才高峰”第七批次项目 （）
