甘肃农业科技Issue(4)：11-16,17,7.DOI:10.3969/j.issn.1001-1463.2015.04.004
来自鹿粪便中1株芽孢杆菌的鉴定及抗真菌肽发酵条件研究
Research on Identification of Bacillus J-2 from Deer Feces and Fermentation Conditions of Antifungal Peptide
摘要
Abstract
In this study, with a strain of bacillus from deer feces as the test strains, we identified it as Bacillus subtilis J-2 by morphology, gram stain, spore stain, physiological and biochemical experiments, and it is analyzed, compared and identified by 16S rDNA sequence at the molecular level. the major factors which impact Bacillus J-2 generating antifungal peptides is analyzed by orthogonal optimization experiments, the optimum fermentation conditions of Bacillus J-2 generating antifungal peptides is derived, in 500 mL erlenmeyer flask is added 150 mL initial pH 7.0 PDL (200) medium access 9%liquid spawn, at the temperature of 37℃for fermentation. 24 h.关键词
芽孢杆菌/鉴定/抗真菌肽/发酵Key words
Bacillus/Identification/Antifungal peptides/Fermentation分类
农业科技引用本文复制引用
翟艺,梁小波..来自鹿粪便中1株芽孢杆菌的鉴定及抗真菌肽发酵条件研究[J].甘肃农业科技,2015,(4):11-16,17,7.基金项目
国家自然科学基金“Rib蛋白在罗伊氏乳杆菌肠道粘附中的功能研究” （）
