临床与病理杂志2015，Vol.35Issue(4)：562-568,7.DOI:10.3978/j.issn.2095-6959.2015.04.009
miR-155在肾结石患者血清和尿液中的表达及其临床意义
Expression and its clinical significance of miR-155 in serum and urine from patients with nephrolithiasis
胡洋洋 1李妍 2李伟 2牛晓振 1彭波 1刘敏1
作者信息
- 1. 同济大学上海市第十人民医院泌尿外科,上海 200072
- 2. 南京医科大学第一临床学院,南京 210000
- 折叠
摘要
Abstract
Objective: hTere may be a potential noninvasive biomarker in miRNAs of both serum and urine, which could predict the chronic kidney disease. In the present study, we will investigate the expression of miR-155 in serum and urine of nephrolithiasis and its clinical signiifcance.Methods: Collected 60 cases blood and urine specimens of patients with nephrolithiasis from August 2011 to August 2012, and collected 50 cases blood and urine specimens of healthy volunteers in comparison. Real time quantity PCR was used to detect the expression of miR-155 in those specimens, and we compare the levels of miR-155 of nephrolithiasis and controls. Estimated glomerular ifltration (eGFR) was calculated at the same time, then analyzed the correlations of miR-155/eGFR and miR-155/CRP levels by simple regression analysis.Results:The expression levels of miR-155 in serum and urine were significance higher in patients with nephrolithiasis than controls. The miR-155 levels inversely correlated with eGFR, and positively correlated with CRP. hTe urinary miR-155 levels signiifcantly and inversely correlated with urinary interleukin(IL)-1β, IL-6 and tumor necrosis factor (TNF)-α levels, but signiifcantly and positively correlated with regulated upon activation, normal T-cell expression, and secreted (RANTES) levels. Conclusion:hTe expression levels of miR-155 in serum and urine of nephrolithiasis patients were signiifcantly elevated, and the elevated levels of miR-155 were correlated with the decrease of eGFR and increase of CRP. Our study suggests miR-155 may play an important role in the pathophysiology of nephrolithiasis, which may be used as a potential predictive biomarker of nephrolithiasis.关键词
肾结石/miR-155/炎症因子Key words
nephrolithiasis/miR-155/inlfammatory cytokine引用本文复制引用
胡洋洋,李妍,李伟,牛晓振,彭波,刘敏..miR-155在肾结石患者血清和尿液中的表达及其临床意义[J].临床与病理杂志,2015,35(4):562-568,7.
