湖南中医药大学学报Issue(5)：23-27,5.DOI:10.3969/j.issn.1674-070X.2014.05.006.023.05
黄芩苷联合黄芩素诱导乳腺癌细胞凋亡的机制研究
Effect and Mechanism of Baicalin and Baicalein Induced Cell Apoptosis in Breast Cancer Cells
摘要
Abstract
Objective To study the breast cancer cell apoptosis is induced by the com bination therapy of baicalin and baicalein and its related m echanism .Methods Different concentrations of baicalin, baicalein and com bined effects of the two drugs in breast cancer MCF-7 cells, test-ing drugs on breast cancer cell viability, apoptosis and apoptosis-related protein expression.Results Baicalin or baicalein acting on the breast cancer cells, cell grow th inhibition was significant-ly higher(P<0.05);com pared with baicalin or baicalein alone, the combination treatm ent with baicalin and baicalein significantly enhanced the anti-proliferative effect (P<0.05).Cell apoptosis was signifi-cantly increased after treating with baicialin or baicalein especially the combination of baicalin and baicalein.Western blot analysis revealed that the expression of Caspase-3, Caspase-9, B ax and p53 were increased.Meanw hile, the level of B cl-2 expression was decreased.Moreover, the activation of p-38 were increased.Conclusions Baicalin and baicalein could induce the apoptosis of breast cancer cells alonely, while the effects significantly enhanced after the combination with baicalin and baicalein. This mechanism may be related with the drugs activate the expression of apoptosis-related protein Caspas-es-3, Caspase-9, Bax and p53, down-regulate the level of Bcl-2, and inhibit the expression of anti-apop-totic protein Bcl-2.关键词
黄芩苷/黄芩素/联合治疗/人乳腺癌/细胞凋亡Key words
baicalin/baicalein/com bination therapy/breast cancer/cells apoptosis分类
医药卫生引用本文复制引用
王婷,黄立中,肖玉洁,罗勇,吴双华..黄芩苷联合黄芩素诱导乳腺癌细胞凋亡的机制研究[J].湖南中医药大学学报,2014,(5):23-27,5.基金项目
高等学校博士学科点专项科研基金资助课题(20114323110006)；湖南省高校创新平台开放基金(湘财教指[2009]70号-09k058)。 （20114323110006）
