吉林大学学报（理学版）Issue(4)：820-824,5.DOI:10.13413/j.cnki.jdxblxb.2014.04.37
Meso-四（对-羟基苯基）卟啉和溶菌酶的相互作用机理
Interaction Mechanism of Meso-tetra(4-hydroxyphenyl)porphyrin and Lysozyme
摘要
Abstract
Meso-tetra(4-hydroxyphenyl)porphyrin (THPP)interacting with lysozyme was investigated by the fluorescence spectroscopy under simulated physiological conditions.The results indicate that THPP quenched the intrinsic fluorescence of lysozyme via a static quenching procedure.The binding constant KA between THPP and lysozyme was 5.97 × 10 4 L/mol at 291 K.According to the thermodynamic parameters,it was obtained that hydrophobic was a main acting force between THPP and lysozyme.The presence of Fe2 + ,Fe3 + ,Cu2 + ,Mg2 + ,Ca2 + and Zn2 + did not affect the binding constant of THPP and lysozyme.According to Förster’s non-radiative energy transfer theory,the distance between THPP and lysozyme was 3.35 nm.The synchronous fluorescence spectra reveal that the vicinity of tryptophan becomes more hydrophobicity with the increase of THPP.关键词
meso-四(对-羟基苯基)卟啉/溶菌酶/荧光猝灭Key words
meso-tetra(4-hydroxyphenyl)porphyrin (THPP)/lysozyme/fluorescence quenching分类
化学化工引用本文复制引用
赵婷婷,毕淑云,王瑜,王天娇,庞博..Meso-四（对-羟基苯基）卟啉和溶菌酶的相互作用机理[J].吉林大学学报（理学版）,2014,(4):820-824,5.基金项目
吉林省自然科学基金(批准号:20140101023JC)、吉林省教育厅“十二五”科技项目(批准号:20140257)和长春师范大学研究生创新基金(批准号:CSCXY2013008) （批准号:20140101023JC）
