河南科技大学学报（医学版）Issue(1)：14-18,5.
氟尿嘧啶诱导大鼠卵巢损伤模型
Establishment of Rat Model of Fluorouracil-induced Premature Ovarian Damage
王盈 1张鹏飞 1王建刚 2席守民 2田晓予1
作者信息
- 1. 河南科技大学第一附属医院，河南洛阳 471003
- 2. 河南科技大学医学院，河南洛阳 471003
- 折叠
摘要
Abstract
Objective To investigate the applicable method and optimal time in establishment of the animal model for fluorouracil-induced ovarian damage. Methods 8-9week-old SD female rats were divided into four groups:normal saline control,low-dose 5-Fu,middle-dose 5-Fu,high-dose 5-FU. The rats in four groups were received daily intraperitoneal injection of normal saline and 5-Fu,1 mL/kg,5 mg/kg,10 mg/kg,20 mg/kg,respectively for two weeks. We detected vaginal smears,wet ovaries wet weighted,and the levels of estradiol(e2),follicle-stimulating hormone(FSH)and anti-Müllerian hormone(AMH)in blood to value the effect of 5-Fu. Results The estrus cycle of rats was remarkably disordered in the BCD groups. It suggested that,compared to A,the level of AMH was significantly decreased(( P <0. 05 )in groups BCD,the level of e2 was also decreased(P<0. 05)in groups CD. While the concentration of FSH was heavily increased after 5-FU administration(P <0. 05). The wet weight and follicle numbers were also decreased(P<0. 05). Conclusion 5-Fu can damage the ovarian and changes the levels of AMH,e2,and FSH. AMH may be more sensitive than e2 and FSH in response to ovarian damage.关键词
氟尿嘧啶/卵巢早衰/大鼠/抗苗勒管激素Key words
5-Fu/premature ovarian failure/rats/anti-mullerian hormone分类
医药卫生引用本文复制引用
王盈,张鹏飞,王建刚,席守民,田晓予..氟尿嘧啶诱导大鼠卵巢损伤模型[J].河南科技大学学报（医学版）,2015,(1):14-18,5.
