山东医药Issue(2)：1-4,4.DOI:10.3969/j.issn.1002-266X.2015.02.001
胃癌细胞 herg mRNA、HERG 蛋白表达及 HERG 电流强度变化
Expression of herg gene, protein and changes of HERG current in gastric cancer cells
摘要
Abstract
Objective To investigate the expression of herg gene , HERG protein and HERG current in gastric cancer cells.Methods Gastric cancer cells and gastric epithelial ( GES) cells were cultured to logarithmic phase .The expres-sions of herg mRNA and HERG protein in gastric cancer cells and GES cells were measured by using RT -PCR and Western blot, respectively.The whole cell configuration of the patch-clamp technique was employed to record HERG currents in va-rious cells .Results HERG mRNA and protein were positively expressed in four gastric cancer cell lines .Expression level of HERG protein in AGS cells was lower compared with the other three gastric cancer cell lines (P<0.05).There was negative expression of herg mRNA and HERG protein in GES cell line .HERG current was detected in gastric cancer cell , whereas there was no HERG current in GES cell .Conclusion Herg gene and HERG protein were highly expressed in gas-tric cancer cells and HERG current was exclusively detected in gastric cancer cells .HERG protein was associated with car-cinogenesis of gastric cancer and may serve as a diagnostic marker for gastric cancer .关键词
胃肿瘤,胃癌/herg基因/HERG蛋白/HERG电流/延迟整流钾通道/肿瘤形成过程Key words
stomach neoplasms/human ether-a-go-go-related gene/HERG protein/HERG current/delayed rectifier potassium channel/neoplastic processes分类
医药卫生引用本文复制引用
邵晓冬,张永国,陈江,王金玲,郭晓钟,任丽楠..胃癌细胞 herg mRNA、HERG 蛋白表达及 HERG 电流强度变化[J].山东医药,2015,(2):1-4,4.基金项目
国家自然科学基金资助项目（30400204）。 （）
