物理学报Issue(2)：1-8,8.DOI:10.7498/aps.64.024301
超声增强藻酸钙凝胶支架材料孔隙率的研究∗
Ultrasonic enhancement of the p orosity of alginate scaffold
摘要
Abstract
Alginate scaffold with a three-dimensional (3D) porous structure can provide sufficient space for the cell to adhere, and has a good biocompatibility and mechanical strength. In this work, low-intensity pulsed ultrasound (LIPUS) is used to enhance the porosity of alginate scaffold based on acoustic cavitation. In the experiment, the alginate-calcium-based 3D scaffold culture system is fabricated with an optimum CaCl2/sodium alginate ratio of 3:5. The mechanical properties of alginate scaffold are measured and scanning electron microscopy is used to analyze the porosity of the scaffold. In addition, the microscopy observation of green fluorescent protein expression and the CCk-8 assessment are adapted to analyze the cell proliferation effect. Experimental results show that with LIPUS treatment under appropriate driving parameters (acoustic pressure 0.055 MPa and treatment time 20 min), the porosity of the 3D scaffold can be significantly improved, which would benefit the cell growth in the scaffold.关键词
低强度超声/空化效应/组织工程支架材料/孔隙率Key words
LIPUS/cavitation/tissue engineering scaffold materials/porosity引用本文复制引用
卢璐,吉鸿飞,郭各朴,郭霞生,屠娟,邱媛媛,章东..超声增强藻酸钙凝胶支架材料孔隙率的研究∗[J].物理学报,2015,(2):1-8,8.基金项目
国家重点基础研究发展计划(批准号:2011CB707900)、国家自然科学基金(批准号:81127901,81227004,81473692,11374155,11174141,11104140,11204144,11274170,11474001,11474161)和江苏省自然科学基金(批准号：BE2011110, BK2012226)资助的课题.@@@@* Projects supported by the National Basic Research Program of China (Grant No.2011CB707900), the National Natu-ral Science Foundation of China (Grant Nos.81127901,81227004,81473692,11374155,11174141,11104140,11204144,11274170,11474001,11474161), and the Natural Science Foundation of Jiangsu Province, China (Grant Nos. BE2011110, BK2012226) （批准号:2011CB707900）
