物理学报Issue(2)：1-6,6.DOI:10.7498/aps.64.028701
高精度实时主动轴向防漂移系统研究∗
A real-time axial activeanti-drift device with high-precision
摘要
Abstract
In a fluorescent nano-resolution microscope based on single molecular localization, drift of focal plane will bring an additional deviation to the accuracy of single molecular localization. Consequently, this will reduce the final resolution of the reconstructed image and cause image degradation. Therefore, it is vital to control the system drift to a minimum level as much as possible. In recent years, the anti-drift ways emerged in endlessly. In this paper we made a systematic study aiming at the method in which optical measurement and negative feedback control are used. The basic principle and its implementation of the system are analyzed, and possible error is also evaluated. Finally, the precision of the system is tested experimentally. With this device, axial drift can be detected and corrected automatically in time, and the axial anti-drift accuracy as high as 9.93 nm can be achieved, which is one order higher than that of the existing commercial microscopies.关键词
纳米分辨/四象限探测器/轴向防漂移Key words
nano-resolution/quadrant detector/axial anti-drift引用本文复制引用
霍英东,曹博,于斌,陈丹妮,牛憨笨..高精度实时主动轴向防漂移系统研究∗[J].物理学报,2015,(2):1-6,6.基金项目
国家重点基础研究发展计划(批准号:2012CB825802,2015CB352005)、国家自然科学基金(批准号:61335001,61235012,61178080,11004136,60878053)、国家重大科学仪器设备开发专项(批准号:2012YQ15009203)和深圳市科技计划(批准号:ZYC201105170233A, JCYJ20120613173049560)资助的课题.@@@@* Project supported by the National Basic Research Program of China (Grant Nos.2012CB825802,2015CB352005), the National Natural Science Foundation of China (Grant Nos.61335001,61235012,61178080,11004136,60878053), the Special Funds of the Major Scientific Instruments Equipment Development of China (Grant No.2012YQ15009203), and the Science and Technology Planning of Shenzhen, China (Grant Nos. ZYC201105170233A, JCYJ20120613173049560) （批准号:2012CB825802,2015CB352005）
