云南师范大学学报（自然科学版）Issue(4)：71-78,8.DOI:10.7699/j.ynnu.ns-2014-059
白芨种子的高效萌发及其无性繁殖体系的构建
Efficient Germination and Establishment of Asexual Propagation System in vitro in Bletilla Striata(Thunb.)Reichb.F
摘要
Abstract
The mature capsule of Bletilla striata was used as material for asymbiotic germination and rapid propagation in this experiment.The seeds was seeding at different culture medium with MS、1/2MS、KC、VW as basal medium added various concentration of 6-BA and coconut milk. The protocorm was inoculating at different propagational media after seed germination and the formation of protocorm. The results showed that the best suitable media for seed germination was liquid media supplementing with 6-BA 1.0 mg·L-1 and NAA 0.2 mg·L-1 ,with highest germination rate.Adding 10% coconut juice could not only improve the seed germination rate, but also increase the growth of protocorm growth and it's propagation.The MS media supple-menting with 6-BA 1.0 mg·L-1 and NAA 0.2 mg·L-1 was best suitable for propagation in vitro,wheres,the MS medium with 6-BA 1.0 mg·L-1 and NAA 0.8 mg·L-1 was best for protocorm and plantlets growth. This protocol of successful plant regeneration by asymbiotic seed germination should permit rapid propagation and conservation of this medicinally important Bletilla striata species.关键词
白芨/无菌播种/原球茎/无性繁殖Key words
Bletilla striata/Seeding in vitro/Protocorm/Asexual propagation分类
农业科技引用本文复制引用
王楷,李玥,张云峰,田宝强,杨秀梅,普家宁,严胜柒..白芨种子的高效萌发及其无性繁殖体系的构建[J].云南师范大学学报（自然科学版）,2014,(4):71-78,8.基金项目
国家自然科学基金资助项目(31060150,31362334) （31060150,31362334）
云南科技厅社会发展科技计划基础研究面上项目(2009CD052)和云南省应用基础研究计划资助项目(2011FA016) （2009CD052）
