中国当代儿科杂志Issue(1)：6-10,5.DOI:10.7499/j.issn.1008-8830.2015.01.002
儿童急性髓系白血病9P21区基因甲基化的研究
Methylation of the genes in the 9P21 region in children with acute myeloid leukemia
摘要
Abstract
Objective To investigate the methylation rate of cyclin-dependent kinase inhibitor 2A (CDKN2A) and cyclin-dependent kinase inhibitor 2B (CDKN2B) in the 9P21 region in children with acute myeloid leukemia (AML) and the association of gene methylation with clinical features and outcomes. Methods The clinical data of 58 children who were newly diagnosed with AML between January 2010 and December 2012 were retrospectively analyzed. Thirty-eight healthy children were recruited as the control group. Genomic DNA was extracted from bone marrow or peripheral blood of the 58 patients and 38 healthy children. The methylation status of CDKN2A and CDKN2B was analyzed by methylation-speciifc multiplex ligation-dependent probe ampliifcation. Results Gene methylation was not found in healthy children. Methylation probes of 44 patients were detected in 58 patients. The methylation of CDKN2A was detected with 136 bp and 237 bp methylation probes. The methylation of CDKN2B was detected with 130 bp, 210 bp, 220 bp, and 417 bp methylation probes. The methylation rate of CDKN2A was 5%, while the methylation rate of CDKN2B was 76%. The methylation detected by some probes was associated with sex, hemoglobin, and platelet count at the first visit. Conclusions The methylation of CDKN2B is a common event in children with AML, while the methylation of CDKN2A is relatively rare.关键词
急性髓系白血病/甲基化/儿童Key words
Acute myeloid leukemia/Methylation/Child引用本文复制引用
张丽,陈晓娟,邹尧,陈玉梅,竺晓凡,阮敏,刘晓明,张家源,郭晔,杨文钰,刘芳,刘天峰,王书春..儿童急性髓系白血病9P21区基因甲基化的研究[J].中国当代儿科杂志,2015,(1):6-10,5.基金项目
国家自然科学基金（81200396）；协和青年科研基金（2012J17）；天津市科技支撑计划（12zcdzsy18100）。 （）
