中国体外循环杂志Issue(1)：1-5,36,6.DOI:10.13498/j.cnki.chin.j.ecc.2015.01.01
血小板microRNAs在先天性心脏病患儿中输血导致的血小板激活作用研究
Impact of packed red blood cell transfusion on platelet activation and aggrega-tion in pediatric patients with cardiac disease
摘要
Abstract
Objective The aim of this study is to observe the impact of packed RBC transfusion on platelet activation and ag⁃gregation in vitro in pediatric patients with cardiac disease and to clarity whether circulating platelet microRNAs could be serve as a new indicator of platelet activation. Methods One hundred infant patients were randomly divided into 2 groups, transfusion group and non-transfusion group. Each group has 50 patients. In vitro transfusions were performed by the addition of RBC obtained from transfusion packs into fresh whole blood with a ratio about 1:4 (0.5 ml of RBC mixed with 1.8 ml of whole blood). After 30 min, the expression of P-selectin and the content of platelet microparticle (PMP) were tested by flow cytometry. Light transmission aggregometry was per⁃formed to determine the platelet aggregation. The expression levels of four kinds of circulation platelet microRNAs were detected by Taq⁃man quantitative real-time PCR. Results There were no significant difference in the baseline hemoglobin level between the two groups ( P>0. 05 ) . After RBC transfusion, the Hb level was elevated by 23 ± 6 g/L. Compared with non-transfusion group, platelet aggregation in transfusion group was significantly increased( P<0.05).Platelet activation was also increased by transfusion as confirmed by the elevation of P-selectin and PMP expressions induced by 20μM ADP. Similar results were found with the four kinds of circulat⁃ing platelet microRNAs ( P<0.05) . In the non-transfusion group, the levels of four kinds of microRNAs in the cyanotic subgroup were significantly elevated than the acyanotic subgroup( P<0.05) . Conclusion RBC transfusion increases in vitro platelet activation in pe⁃diatric patients with cardiac disease, providing a possible explanation for the increase in recurrent ischemic event and mortality reported after RBC transfusion in clinical practice. Circulation platelet microRNAs may serve as a new marker of platelet activation.关键词
输血/血小板激活/microRNAs/先天性心脏病Key words
Transfusion/Platelet activation/MicroRNAs/Congenital heart disease引用本文复制引用
苗晓蕾,王旭,刘晋萍,崔勇丽,赵明霞,冯正义,赵举,龙村,李守军,晏馥霞..血小板microRNAs在先天性心脏病患儿中输血导致的血小板激活作用研究[J].中国体外循环杂志,2015,(1):1-5,36,6.基金项目
首都临床特色应用研究 （）
