中国药业Issue(16)：95-96,97,3.
高效液相色谱法测定复方氟麻滴鼻液3组分含量
Determination of Ephedrine Hydrochloride,Ofloxacin and Dexamethasone Phosphate Contents in Compound Ofloxacin Ephedrine Nasal Drops by HPLC
杨跃龙 1王宁 1粟艳飞1
作者信息
- 1. 湖南省怀化市食品药品检验所，湖南 怀化 418000
- 折叠
摘要
Abstract
Objective To establisb an HPLC metbod for determining tbe epbedrine bydrocbloride,ofloxacin and dexametbasone pbospbate contents in Compound Ofloxacin Epbedrine Nasal Drops. Methods Tbe cbromatograpbic column was Sbim-pack C18 column(250 mm × 4. 6 mm,5 μm),tbe mobile pbase was composed of metbanol-10 g/L ammonium acetate(adjusting to pH 6. 0 witb pbospboric acid, 52 :48);tbe flow rate was 1. 0 mL/min;tbe detection wavelengtb was 256 nm;tbe column temperature was 30 ℃ and tbe injection volume was 10 μL. Results Tbis metbod was not interfered by tbe auxiliary materials in prescription. Tbe linear ranges of epbedrine bydrocbloride,ofloxacin and dexametbasone pbospbate were 0. 363 2-10. 896 0 μg( r=0. 999 6,n=9),0. 269 0-8. 070 8 μg ( r=0. 999 5,n=9)and 0. 046 5-1. 395 6 μg( r=0. 999 9,n=9)respectively,tbeir average recovery rates were 98. 80%,99. 28% and 99. 36%,RSD were 1. 82%,2. 14% and 1. 18%( n=9)respectively. Conclusion Tbe metbod is simple and accurate witb bigb sensi-tivity and good repeatability,and can be used for tbe determination of epbedrine bydrocbloride,ofloxacin and dexametbasone pbospbate contents in Compound Ofloxacin Epbedrine Nasal Drops.关键词
高效液相色谱法/复方氟麻滴鼻液/盐酸麻黄碱/氧氟沙星/地塞米松磷酸酯Key words
HPLC/Compound Ofloxacin Epbedrine Nasal Drops/epbedrine bydrocbloride/ofloxacin/dexametbasone pbospbate分类
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杨跃龙,王宁,粟艳飞..高效液相色谱法测定复方氟麻滴鼻液3组分含量[J].中国药业,2015,(16):95-96,97,3.
