癌变·畸变·突变Issue(3)：183-189,7.DOI:10.3969/j.issn.1004-616x.2013.03.005
不同中医临床证型下行型鼻咽癌组织细胞核蛋白质差异表达的初步研究
Different nuclear protein expression in downward progressive nasopharyngeal carcinoma
摘要
Abstract
OBJECTIVE: To study the different nuclear protein expression in primary focus of downward progressive type of nasopharyngeal carcinoma in patients with different clinical patterns according to TCM classification. METHODS:Nuclear proteins profiles in fire-toxin stagnation type,Qi-yin deficiency type and Qi-blood coagulation type were measured by two-dimensional gel electrophoresis and matrix assisted laser. RESULTS:Compared with the expression of nuclear protein spots from patients with Qi-yin deficiency type,there were 28 up-regulated protein spots and 12 down-regulated protein spots from patients with Qi-blood coagulation type. We also found 9 up-regulated protein spots and 19 down-regulated protein spots from patients with fire-toxin stagnation type. Compared with the expression of nuclear protein spots from patients with Qi-blood coagulation type,there were 25 up-regulated protein spots and 18 down-regulated protein spots from patients with fire-toxin stagnation type. Three differential proteins were identified successfully by MALDI-TOF-MS. Up-regulated proteins in one spot were heterogeneous nuclear ribonucleoprotein H (hnRNP H) and tubulin beta-1 chain (TUB1) from patients with Qi-blood coagulation type and the other down-regulated protein spot was type VI collagen alpha 2 chain (COLⅥA2) precursor. CONCLUSION:Heterogeneous nuclear ribonucleoprotein H (hnRNP H) and tubulin beta-1 chain (TUB1) in nasopharyngeal carcinoma tissues from patients with Qi-blood coagulation type were up-regulated,type VI collagen alpha 2 chain precursor in those tissues from patients with fire-toxin stagnation type was down-regulated.关键词
下行型鼻咽癌/中医证型/核蛋白质/差异表达Key words
downward progressive type of nasopharyngeal carcinoma/patterns in terms of TCM/nuclear protein/different expressions分类
医药卫生引用本文复制引用
何迎春,刘宇勤,廖端芳,戴娜,何兰,范婧莹,田道法..不同中医临床证型下行型鼻咽癌组织细胞核蛋白质差异表达的初步研究[J].癌变·畸变·突变,2013,(3):183-189,7.基金项目
国家自然科学基金(81273988，81072904)；中国博士后基金(2011M501277)；湖南省外国专家局引智项目(湘财社指[2011]民40号，湘教通[2012]488号)；湖南省中医五官科学重点学科项目 （81273988，81072904）
