癌变·畸变·突变Issue(3)：198-200,204,4.DOI:10.3969/j.issn.1004-616x.2013.03.008
ENU致大鼠体内Pig-a基因突变的时-效关系和量-效关系研究
Time-course and dose-response relationship study of ENU-induced Pig-a gene mutation in the rat
摘要
Abstract
OBJECTIVE: To study time-course and dose-response relationship of ENU-induced rat phosphatidylinositol glycan class-A(Pig-a) gene mutation and explore the potential of this assay to be integrated into repeat dose study,and to establish a rat Pig-a gene mutation assay. MEHTODS:Fifteen rats were randomly assigned to be treated with PBS and ENU (20 mg/kg and 40 mg/kg) for 3 consecutive days by oral gavage. Jugular blood samples were collected on days-1(the day before administration),15,30 and 45. Erythrocytes were enriched and incubated with Anti-CD59-PE and nucleic acid dye solution,and then analyzed with flow cytometer. RESULTS:On days 15,30 and 45,the Pig-a gene mutation frequencies in RBCs and RETs in low and high dosage groups were elevated significantly compared with that in control group (all P values <0.01),and the mean frequencies in high dosage group were about 2-3 times of that in low dosage group. The Pig-a gene mutation frequencies were maintained at high levels during days 15-45. CONCLUSION:The in vivo Pig-a gene mutation assay was established successfully in rats. Time-course results suggested that this assay can be integrated into repeated-dose study.关键词
Pig-a基因/突变/时-效关系/量-效关系Key words
Pig-a gene/mutation/time-course relationship/dose-response relationship分类
医药卫生引用本文复制引用
张铭,周长慧,王征,王庆利,常艳..ENU致大鼠体内Pig-a基因突变的时-效关系和量-效关系研究[J].癌变·畸变·突变,2013,(3):198-200,204,4.基金项目
国家“十二五”重大专项基金资助项目(2012ZX09505-001-003) （2012ZX09505-001-003）
