癌变·畸变·突变Issue(3)：201-204,4.DOI:10.3969/j.issn.1004-616x.2013.03.009
大蒜素对人食管癌EC9706细胞凋亡的诱导作用
The induction of garlicin in human esophageal cancer cell EC9706 apoptosis
摘要
Abstract
OBJECTIVE: To study the apoptotic effect of garlicin on esophageal cancer EC9706 cells. METHODS: We used 18 μg/mL garlicin to treat esophageal cancer EC9706 cells,and analyzed the effect by cell culture,cell count,flow cytometry analysis,Hoechst33258 staining,HE staining and electron microscopy. RESULTS:Proliferation of EC9706 cells could be inhibited by 18μg/mL garlic,cell growth inhibition rate was 75.89%. Flow cytometry analysis showed that garlic could induce the emergence of apoptosis,and the rate was 23.8%,blocked at G2/M phase. Concentrated cell nucleus appeared granular and fluorescent by Hoechst33258 staining. Light microscope and electron microscope showed that the morphology of the cells treated with 18 μg/mL garlic appeared shrunken,cell nucleus concentrated,chromatin agglutination,mitochondria swelling,and other significant apoptosis characteristics. CONCLUSION:This study suggested that garlic could induce apoptosis of esophageal cancer EC9706 cells effectively.关键词
大蒜素/人食管癌/EC9706细胞/诱导作用Key words
garlicin/human esophageal cancer/EC9706 cells/induction role分类
医药卫生引用本文复制引用
陈兰英,宋巍,杨海波,于淮滨,刘亚军,支双成,杨峰..大蒜素对人食管癌EC9706细胞凋亡的诱导作用[J].癌变·畸变·突变,2013,(3):201-204,4.基金项目
河南省重点科技攻关项目(092102310006)，河南省教育厅基金项目(2009B180001) （092102310006）
