癌变·畸变·突变Issue(4)：280-284,5.DOI:10.3969/j.issn.1004-616x.2013.04.008
人参多糖与他莫昔芬联合对人乳腺癌MCF-7细胞凋亡的影响及其机制研究
The effect and mechanism of ginseng polysaccharide on tamoxifen-induced apoptosis in human breast cancer MCF-7 cells
刘文娟 1高鹏 2王大海2
作者信息
- 1. 哈尔滨医科大学附属第二医院感染内科，黑龙江 哈尔滨 150081
- 2. 哈尔滨市儿童医院心脏外科，黑龙江 哈尔滨 150010
- 折叠
摘要
Abstract
OBJECTIVE: To study the effect and mechanism of ginseng polysaccharide (GPS) on tamoxifen (TAM)-induced apoptosis in human breast cancer cells. METHODS:Human breast cancer cells MCF-7 were treated with different doses of GPS for 48 h. MTT assay was used to evaluate the minimal effective GPS dose. Then cells were treated with GPS at minimal effective dose combined with different doses of TAM. Bürgi formula was used to assess the synergy effect between GPS and TAM. Cellular mitosis was detected by Giemsa staining assay. Apoptosis was studied by DAPI staining analysis and flow cytometer. The expression of Fas,Caspase-9 and Parp was measured by Western blot. RESULTS:GPS at 40μg/mL was the minimal effective dose on MCF-7 cells. Combined treatment of GPS at 40μg/mL and TAM at 1 μg/mL showed the best effect in cell growth inhibition (q=1.82). Combination of GPS and TAM synergistically enhanced apoptosis of MCF-7 in comparison with GPS or TAM alone (q=2.19,P<0.05). Combinated treatment of GPS and TAM significantly inhibited cellular mitosis compared with GPS or TAM alone (P<0.05). Treatment of GPS+TAM induced increased level of Fas as well as cleavage of Caspases-9 and Parp. CONCLUSION:Combined treatment of GPS and TAM induced apoptosis in MCF-7 cell through Fas signal in a synergetic manner.关键词
人参多糖/他莫昔芬/凋亡/MCF-7细胞Key words
ginseng polysaccharide/tamoxifen/apoptosis/MCF-7 cells分类
医药卫生引用本文复制引用
刘文娟,高鹏,王大海..人参多糖与他莫昔芬联合对人乳腺癌MCF-7细胞凋亡的影响及其机制研究[J].癌变·畸变·突变,2013,(4):280-284,5.
