癌变·畸变·突变Issue(5)：341-346,6.DOI:10.3969/j.issn.1004-616x.2015.05.003
Toll样受体2基因多态性与胃癌及EB病毒相关胃癌易感性的关系
TLR2 gene polymorphism and susceptibility to gastric carcinoma and EBV-associated gastric carcinoma
摘要
Abstract
OBJECTIVE:The aim of our study was to evaluate the potential associations between the single nucleotide polymorphisms (SNPs) of Toll-like receptor (TLR2) rs3804099 and rs3804100 genes and the risk of gastric carcinoma(GC),especially to Epstein-Barr virus-associated gastirc carcinoma (EBVaGC).METHODS:TLR2 rs3804099 and rs3804100 gene polymorphism were assessed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 185 cases of EBV-negative GC,41 cases of EBVaGC. 100 cases of peripheral blood samples from healthy individuals were also examined. The data was analysed by SPSS.RESULTS:As for theTLR2 gene (rs3804099), there was significant difference between the GC group and the control group in both genotype and allelic 22frequencies (χ=5.617,P=0.018;χ=6.467,P=0.011). The C allele and C allele carriers frequencies of gastric carcinoma 22group were significantly higher than those of controls (χ=6.467,P=0.011;χ=4.444,P=0.035). Compared with the wild type TT, the CC genotype could increase the risk of gastric cancer (OR=3.554,95%CI=1.179-10.715). As for theTLR2 gene (rs38040100),there were no significant differences between the GC group and the control group in genotype,C allelic frequency and C allele carrier frequency (P>0.05). In all the indicators,no polymorphism was found to be related to EBVaGC in the studied population (P>0.05).CONCLUSION:There was an association betweenTLR2 rs3804099 gene polymorphism and gastric carcinoma,and the C allele may be a risk factor for gastric carcinoma. Carrying the C allele may increase the risk of gastric carcinoma. No association was observed betweenTLR2 (rs3804099 and rs38040100) gene polymorphism and EBVaGC.关键词
胃癌/EB病毒/ToI样受体/单核苷酸多态性Key words
gastric carcinoma/Epstein-Barr virus/Toll-like receptor/single nucleotide polymorphism分类
医药卫生引用本文复制引用
卫美蓉,刘颂,赵真真,王笑峰,罗兵..Toll样受体2基因多态性与胃癌及EB病毒相关胃癌易感性的关系[J].癌变·畸变·突变,2015,(5):341-346,6.基金项目
国家自然科学基金(30970157)；山东省自然科学基金(ZR2011CM 016) （30970157）
