癌变·畸变·突变Issue(5)：347-352,6.DOI:10.3969/j.issn.1004-616x.2015.05.004
泛素连接酶RING2对苯并[a]芘染毒人支气管上皮细胞周期和P53蛋白表达的影响
Role of ubiquitin protein ligase RING2 in cell cycle and expressions of P53 in human bronchial epithelial cells exposed to benzo[a]pyrene
摘要
Abstract
[ABSTRACT]OBJECTIVE:To investigate the role of ubiquitin protein ligase RING2 in cell cycle and expression of P53 in human bronchial epithelial cells exposed to benzo[a]pyrene.METHODS:The untreated 16HBE cellsgroups were used as negative control groups,the DMSOgroups were used assolvent control groups,the MOCK groups were used as sequence control groups. 16HBE cells and 16HBE(siRNA-RING2) cells were exposed to BaP at different concentrations (1,2,4,8,16,32μmol/L) for 24 h,and were exposed to BaP at 16μmol/L for different time points (1,2, 4,8,12,24 h). Flow cytometry was used to evaluate the cell cycle. The levels of P53 protein were detected by Western-blot.RESULTS:After BaPtreatment,compared with the negative control groups,ateach concentration and each time point the proportion of S phaseof 16 HBE cellswere significantly increased (P<0.01). However at each concentration and each time point 16HBE (siRNA-RING2) cells groups the proportion of S phase cells were significantly decreased (P<0.01). Covariance analysis shows grouping factors (with or withoutRNAi) andall concentrations had impact on the proportion of S phase,P values were both less than 0.01. The estimated mean of the proportion of S phase in 16HBE (siRNA-RING2) cell group (17.09%) was significantly lower than that of 16HBE cell group (31.55%). Grouping factors (with or without RNAi) and the exposure time all have impacted on theproportion of S phase,P values were both less than 0.01. The estimated mean of the proportion of S phase in 16HBE (siRNA-RING2) cell group (13.07%) was significantly lower than that of 16HBE cell group (28.04%) (P<0.05). After BaPtreatment,compared with the negative control group,ateach concentration and each time pointP53 protein levels at16 HBE cell groups were significantly increased (P<0.05). However,ateach concentration and each time point 16HBE (siRNA-RING2) cell groups (except16μmol/LBaP treated 8 h) the levels of P53 protein were significantly decreased (P<0.05). Covariance analysis shows grouping factors (with or without RNAi) and all concentration showed impact on the level of P53 protein,P values were 0.026 and 0.028,respectively. The estimate mean ofthe levels of P53 protein in 16HBE (siRNA-RING2) cell group (0.989) was significantly lower than thatof 16HBE cell group (1.375) (P<0.05). Grouping factors (with or without RNAi) and the exposure time both impacted on the level of P53 protein,P values were 0.007 and 0.035,respectively. The estimate mean of the levels of P53 protein in 16HBE (siRNA-RING2) cell group (0.857) was significantly lower than that of 16HBE cell group (1.541) (P<0.05).CONCLUSION:The histone ubiquitination modifications in which RING2wasinvolved may regulate DNA repair by affecting the expression of P53 and cell cycle S phase changes.关键词
苯并a芘/人支气管上皮细胞/泛素连接酶RING2/细胞周期/P53蛋白Key words
benonzo[a]pyrene/human b ronchial e pithelial c ell/ubiquitin p rotein l igase R ING2/cell c ycle/P53分类
医药卫生引用本文复制引用
范燕峰,陈文涛,王武斌,杨瑾..泛素连接酶RING2对苯并[a]芘染毒人支气管上皮细胞周期和P53蛋白表达的影响[J].癌变·畸变·突变,2015,(5):347-352,6.基金项目
国家自然科学基金项目(81273041，30901180) （81273041，30901180）
