癌变·畸变·突变Issue(5)：378-382,5.DOI:10.3969/j.issn.1004-616x.2015.05.011
乙二胺为核心的PAMAM树枝状聚合物（5.0代）纳米材料溶液的遗传毒性研究
Genotoxicity of PAMAM dendrimer (ethylenediamine core) generation 5.0 solution
摘要
Abstract
OBJECTIVE:To study the genotoxicity of PAMAM dendrimer (ethylenediamine core) generation 5.0 solution.METHODS:In the plate incorporation test ofSalmonella typhimurium,the average colony number of spontaneous revertants of TA97,TA98,TA100,TA102,TA1535 were set at 5 concentrations:3 985,797,139.4, 31.88 and6.376µg per plate. In the cytokinesis-block micronucleus cytome(CBMN Cyt) assay,the final concentration 0.625,1.25,2.5,5,10µg/mL of PAMAM dendrimer (ethylenediamine core) generation 5.0 wasadded to L5178Y cells,then assessed the micronucleus cytome effect,dose-effect and time-effect relationships.RESULTS: The colony number of revertants for the materials tested did not obviously increase,compared with that of spontaneous revertants. The result of Ames test for the subject was negative. In the CBMN Cyt assay,the frequency of nuclear buds increased at concentration of 1.25µg/mL.The formation of total,type Ⅰ andtypeⅡ micronuclei as well as nuclear-cytoplasmic bridges were oberservedat andabove concentration of 2.5µg/mL. At of 5µg/mL,the time-effect results indicated that the number of total,type Ⅰ amdtype Ⅱ micronuclei and nuclear buds increased at 9 h,while the nucleoplasmic bridges started to increase at 18h. The indexes of total,type Ⅰandtype Ⅱ micronuclei,nucleoplasmic bridges and nuclear buds reached their peak at 27 h.CONCLUSION:The PAMAM dendrimer (ethylenediamine core) generation 5.0 solution showed no potential mutagenicity. While could cause four kinds of micronucleus cytome effects with clear dose-effect and time-effect in L5178Y cells. From the dose and time point of view,nuclear bud was the most sensitive index.关键词
树枝状聚合物/Ames试验/遗传毒性/微核组学效应Key words
dendrimers/Ames test/genotoxicity/micronucleus genomic effect分类
医药卫生引用本文复制引用
刘甜甜,沈春琳,吕路路,张天宝..乙二胺为核心的PAMAM树枝状聚合物（5.0代）纳米材料溶液的遗传毒性研究[J].癌变·畸变·突变,2015,(5):378-382,5.基金项目
国家自然科学基金资助项目(81273104) （81273104）
