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红曲霉发酵农副产品过程中多酶特性的初步研究

蒋汶 汤文晶 郑天柱 谭胜 张庆庆

安徽工程大学学报Issue(5):1-7,7.
安徽工程大学学报Issue(5):1-7,7.

红曲霉发酵农副产品过程中多酶特性的初步研究

Preliminary study on multi enzyme properties of Monascus in agricultural products fermentation process

蒋汶 1汤文晶 1郑天柱 1谭胜 1张庆庆1

作者信息

  • 1. 安徽工程大学 生物与化学工程学院,安徽 芜湖 241000
  • 折叠

摘要

Abstract

Agricultural and sideline products as rice flour,bran,bean dregs were used as the main nutrient medium with the strain of Monascus ZL307.The conditions had been optimized by orthogonal experimen-tal design.The results showed that amylase activity peaked at 1 63.327 5 U/mL in 2 days.Protease activi-ty peaked at 92.249 8 U/mL in 3 days.Esterifying enzyme activity peaked at 1 6.672 5 U/mL in 7 days. Filter paper activity peaked at 0.132 2 U/mL,endonuclease activty peaked at 0.333 1 U/mL,exonuclease activity peaked at 0.134 7 U/mL andβ-glucosidase activity peaked at 0.153 8 U/mL in 8 days.Tolerance to temperature of four enzymes were cellulase>amylase> proteinase> esterase.Tolerance to pH were cellulase>protease>amylase>esterase.

关键词

红曲霉/液态发酵/多酶特性/正交优化

Key words

Monascus/liquid fermentation/multi-enzyme properties/orthogonal optimization

分类

生物科学

引用本文复制引用

蒋汶,汤文晶,郑天柱,谭胜,张庆庆..红曲霉发酵农副产品过程中多酶特性的初步研究[J].安徽工程大学学报,2015,(5):1-7,7.

基金项目

国家大学生创新创业训练计划基金资助项目(201310363021) (201310363021)

安徽工程大学学报

2095-0977

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