按摩与康复医学Issue(3)：176-180,5.
脂肪干细胞分化及贴覆支架能力的研究
Differentiation and Adhesion Ability of Adipose-derived Stem Cells
刘一亮 1赵劲民 1苏伟 1秦义武1
作者信息
- 1. 广西医科大学一附院创伤骨科，广西南宁530021
- 折叠
摘要
Abstract
Objective:To observe the differentiation and adhesion ability of adipose-derived stem cells (ADSCs). Methods:The ADSCs were isolated from rabbit cervical subcutaneous fat and puri-fied by enzyme digestion. The growth situation of ADSCs was observed under microscope. Adding induced nutrient solution to the cells to induce osteogenesis and adipogenesis. The passage cells were stained by Alkaline phosphatase、Calcium salt deposition and oil red O staining associated staining identification. Cells and scaffold composite situation were observed under the electron mi-croscopic. Results:In the induction condition,Alkaline phosphatase and calcium salt deposits were positive expression, Oil red O stain positive expression. Conclusions:ADSCs have multipotential differentiation potential,it can cover and grow on the bracket well.关键词
脂肪干细胞/诱导分化/细胞培养/组织工程Key words
adipose derived stem cells (ADSCs)/induced differentiation/cell culture/tissue en-gineering分类
医药卫生引用本文复制引用
刘一亮,赵劲民,苏伟,秦义武..脂肪干细胞分化及贴覆支架能力的研究[J].按摩与康复医学,2013,(3):176-180,5.
