北华大学学报（自然科学版）Issue(6)：729-732,4.DOI:10.11713/j.issn.1009-4822.2015.06.007
NS特异性干扰载体pGenesil-1-NS的构建及鉴定
Construction and Identification of pGenesil-1-NS Interference Vector
摘要
Abstract
Objective Nucleostemin,a protein to maintain the proliferations of stem cell and cancer cell, may become a potential target for tumor gene therapy. This study aimed to construct the NS specific interference vector pGenesil-1-NS,and to lay a foundation for further study. Method Based on the published NS-mRNA sequence (NM_206825),5′-AAGCCTA GGAAAGACCCAGG-3′(397-416) was selected as candidate target sequence. According to the shRNA carrier design principle,shDNA that could be transcripted to shRNA was designed and synthesized,then inserted into pGenesil-1 vector. The recombinant was identified by restriction enzyme digestion analysis followed by sequence analysis. Results Confirmed by restriction analysis and sequencing,shDNA was correctly inserted into the vector. Conclusion NS-target interference vector pGenesil-1-NS was successfully constructed,which would be applied in the study of NS function in tumor generation and progression.关键词
核干细胞因子基因/pGenesil-1载体/发夹状RNA/RNA干扰Key words
nucleostemin gene/pGenesil-1 vector/shRNA/RNAi分类
生物科学引用本文复制引用
高鸿霞,王国庆,刘燕,霍中华,李钰..NS特异性干扰载体pGenesil-1-NS的构建及鉴定[J].北华大学学报（自然科学版）,2015,(6):729-732,4.基金项目
吉林省卫生厅基金项目(2012Z032/2014Z077) （2012Z032/2014Z077）
吉林市科技创新服务平台项目(2013625027) （2013625027）
