北华大学学报（自然科学版）Issue(6)：733-736,4.DOI:10.11713/j.issn.1009-4822.2015.06.008
长白山2种橐吾属植物乙醇提取物抗肿瘤作用研究
On Antitumor Effects of Ethanol Extracts from 2 Kinds of Ligularia Genus Plants in Changbai Mountain
摘要
Abstract
Objective To discuss the antitumor effects of ethanol extracts from Ligularia jaluensis and Ligularia intermedia in vitro,and to provide references for the development of antitumor drugs as well as the comprehensive utilization and development of Ligularia genus resources in Changbai Mountain. Method The inhibiting effects of ethanol extracts from Ligularia jaluensis and Ligularia intermedia on the multiplications of 2 kinds of tumour cells (Hela,A549) were determined by MTT colorimetric assay in vitro. Flow cytometry was applied to test cell apoptosis. The effects of ethanol extracts from two kinds of ligularia genus plants on the clearance of Hydroxy free radical( ) and superoxide anion free radical ( O-2 · ) were measured by Phenanthroline and Pyrogallol autoxidation methods. Results The A549 and Hela cells were treated with different concentrations of the ethanol extracts for 48 hours, and the inhibition rates increased remarkably with increasing of concentration. For the clearance of ,the IC50 of FXEtOH,XBEtOH and Vc were 73. 34,335. 30 and 79. 31μg/mL,respectively. For the clearance of O-2 ·, the IC50 of FXEtOH, XBEtOH and Vc were 64. 81, 131. 89 and 191. 62 μg/mL, respectively. Conclusion Both the ethanol extracts of Ligularia jaluensis and Ligularia intermedia possess the antineoplastic activity in vitro and good comprehensive antioxidant capacity which may be one of their possible mechanism on antitumor.关键词
复序橐吾/狭苞橐吾/抗肿瘤/抗氧化Key words
Ligularia jaluensis/Ligularia intermedia/anti-tumor/antioxidant分类
医药卫生引用本文复制引用
朱梅,孙海明,任桂红,赵桂云,董然,刘洪章..长白山2种橐吾属植物乙醇提取物抗肿瘤作用研究[J].北华大学学报（自然科学版）,2015,(6):733-736,4.基金项目
吉林省科技发展计划项目(20100254) （20100254）
吉林省教育厅科学技术研究项目(2012138) （2012138）
