工程科学学报2015，Vol.37Issue(11)：1410-1414,5.DOI:10.13374/j.issn2095-9389.2015.11.003
一株碱性产氨浸铜细菌改良试验研究
Improvement experiment study of alkaline copper leaching bacteria
摘要
Abstract
Alkaline leaching bacteria are new efficient strains for processing alkaline minerals. Slurry gradient domestication and ultraviolet mutagenesis were introduced to improve the adaptive capacity to environment and leaching rate of the strains. With the help of Design Expert-8 software, a three-factor three-level Box-Behnken experiment was conducted to investigate the influence of pulp density, initial bacteria inoculation and temperature on the leaching rate. The optimal experimental condition was determined by Design Expert-8 software and a prediction model was proposed in this paper. Comparative experiment was conducted to investigate the differences before and after this improvement experiment under the optimal conditions. The results show that the improved strain can adapt to the pulp condition better. The population of the improved strain can up to 6. 5 × 108 mL-1 and the leaching rate reaches to 50. 57%, while the population of the original strain can up to 3 × 108 mL-1 and the leaching rate reaches only to 29. 03%.关键词
铜矿处理/微生物浸矿/细菌/驯化/诱变/菌种改良Key words
copper ore treatment/microbial leaching/bacteria/domestication/mutagenesis/strain improvement分类
矿业与冶金引用本文复制引用
胡凯建,王洪江,李广泽,吴爱祥,黄明清..一株碱性产氨浸铜细菌改良试验研究[J].工程科学学报,2015,37(11):1410-1414,5.基金项目
国家自然科学基金资助项目(50934002, 51304011) （50934002, 51304011）
博士学科点专项科研基金资助项目(20110006130003) （20110006130003）
