北京中医药大学学报2015，Vol.38Issue(10)：686-689,702,721,6.DOI:10.3969/j.issn.1006-2157.2015.10.009
益气活血解毒中药对胶原诱导性关节炎大鼠NF-κB信号通路相关因子表达的影响
Effects of Yiqi Huoxue Jiedu Fang on gene expression of cytokines involving NF-κB signal pathway in collagen II-induced arthritic rats
摘要
Abstract
Objective To study the gene expressions of key cytokines involving NF-κB signaling pathway in collagen II-induced arthritic ( CIA ) rats treated with Yiqi Huoxue Jiedu Fang ( qi-tonifying blood-activating and toxin-removing formula) .Methods We used collagen II-induced arthritic ( CIA) rats by bovine II collagen as an animal model and Leflunomide as controls.CIA rats received treatment with Yiqi Huoxue Jiedu Fang.The levels of inflammatory cytokines in serum were measured with protein chip assay, and hematoxylin dyeing was used to observe the histopathological changes.The gene expressions of the key cytokines in NF-κB signal pathway were detected by real-time PCR.Results This formula inhibited the proliferation of synovial tissues, reduced inflammatory exudation and narrowed the range of fibroid necrosis.It also down-regulated inflammatory cytokines ( TNF, IL-1, IL-5, IL-6 and IL-10) in serum of CIA rats.The gene expressions of NF-κB and IKK decreased in CIA rats treated with this formula.Conclusion Yiqi Huoxue Jiedu Fang could regulate the expressions of inflammatory cytokines and the key factor of NF-κB signal pathway for inhibiting inflammatory reaction in injured tissues, restoring the joint injury.Those could be the key active mechanism of RA treated with this formula.关键词
益气活血解毒中药/胶原诱导性关节炎/炎症细胞因子/NF-κB信号通路/大鼠Key words
Yiqi Huoxue Jiedu Fang/collagen II-induced arthritis/inflammatory cytokines/NF-κB sig-nal pathway/rats分类
医药卫生引用本文复制引用
董玢,娄利霞,韦翊丽,金秋硕,柴立民..益气活血解毒中药对胶原诱导性关节炎大鼠NF-κB信号通路相关因子表达的影响[J].北京中医药大学学报,2015,38(10):686-689,702,721,6.基金项目
国家自然科学基金资助项目( No.81173228) （ No.81173228）
