吉林大学学报（医学版）Issue(6)：1230-1234,5.DOI:10.13481/j.1671-587x.20150625
ELOVL2基因多态性与乳母乳汁 DHA 水平的关联性分析
Association analysis on polymorphisms of ELOVL2 gene and DHA level in breast milk of lactating mothers
摘要
Abstract
Objective To investigate the association between rs2281951 and rs3798753 in ELOVL fatty acid elongase2 (ELOVL2 gene)and the docosahexenoic acid (DHA)level in breast milk,and to clarify the influence of the polymorphisms of ELOVL2 gene in the DHA level of breast milk.Methods 209 healthy maternals were selected and signed the consent form and completed the 3-day 24-hour dietary recall questionaire on one day during the 22nd-the 25th day after partum,and 20 mL breast milk was collected.The DHA level in breast milk was detected with gas chromatography.The milk DNA was extracted and two single nucleotide polymorphisms (SNPs) of ELOVL2 gene were detected by Sequenom Mass Array System. UNPHASED 3.012 genetics software was adopted to analyze the quantitative trait of haplotype and the DHA level in breast milk.Results The distribution of genotypic frequencies of rs2281591 and rs3798713 sites in ELOVL2 gene was consistent with Hardy-Weinberg equilibrium (P > 0.05).The dietary fatty acid intakes and the milk DHA levels of maternals carrying different genotypes had no statistically significant differences (P > 0.05 ). The DHA levels in breast milk of maternals carring different rs3798713 (CG)-rs2281591 (AG)haplotypes had no statistically significant difference (χ2 =3.422,df =5,P =0.635).Conclusion Rs3798713 and rs2281951 and constructed haplotypes in ELOVL2 gene are not related to the DHA levels in breast milk.关键词
基因多态性/单倍型/ELOVL2 基因/乳汁/二十二碳六烯酸Key words
gene polymorphism/haplotype/ELOVL2 gene/breast milk/docosahexenoic acid分类
医药卫生引用本文复制引用
李响,丁珍,吴义霞,陈雪妍,刘国良,谢林..ELOVL2基因多态性与乳母乳汁 DHA 水平的关联性分析[J].吉林大学学报（医学版）,2015,(6):1230-1234,5.基金项目
国家自然科学基金资助课题 （）
