滨州医学院学报Issue(5)：327-330,4.
血清浓度对心肌细胞自噬的影响磁
The effect of different serum concentrations on cardiomyocytes'autophagy
摘要
Abstract
Objective To investigate the effect of different serum concentrations on cardiomyocytes'autophagy .Methods Rat embryonic cardiac cell line H9c2 was taken as specimens to detect the levels of cellular autophagy in three time points of 6 ,12 , 24 h and four different concentrations of 0 ,2 ,5 ,10% .Western Blot was used to detect the changes of cardiac cell LC 3 Ⅱand P62 ,and acridine orange staining was used to detect acidic vesicles .Results Before 6 h ,10% concentrated serum had the low‐est level of autophagy .During 6~12 hours ,autophagy was promoted with the increasing of serum concentrations .After 24 h , serum‐deprived group promoted autophagy .Adding 2 ,5 ,10% serum could inhibit autophagy .Conclusion To find out the fac‐tors of promoting autophagy ,the models with lower autophagy should be selected .Within 6 hours ,10% concentrated serum cultured cardiac cells are the first choice .During 6~12 hours ,serum‐deprived culture system should be used .After 24 hours , serum culture system can be used .关键词
心肌细胞/细胞培养/自噬/P62/LC3ⅡKey words
cardiomyocytes/cell culture/autophagy/P62/LC3Ⅱ分类
医药卫生引用本文复制引用
刘平平,李文静,常芬,赵静,董以雷..血清浓度对心肌细胞自噬的影响磁[J].滨州医学院学报,2015,(5):327-330,4.基金项目
项目国家自然科学基金 （）
