草业科学Issue(10)：80-87,8.DOI:10.11686/cyxb2014470
不同苜蓿品种对豌豆蚜中肠消化酶活性的影响
The effect of different alfalfa varieties on the midgut protease activity of pea aphid (Acyrthosiphon pisum)
摘要
Abstract
This research measured the midgut protease enzyme activity in the red and green pea aphid (Acyrtho-siphon pisum )after they had fed on 8 alfalfa (Medicago sativa )varieties with differing resistance.The midgut protease activity of the red pea aphid was (9.71 ±0.63)U/mL after they had fed on the variety Weinan,and was (0.33 ±0.10)U/mL after feeding on variety Sandili.The protease activity of the green pea aphid was (9.88±0.56)U/mL after feeding on the variety Hunter river,and (0.43±0.02)U/mL after feeding on Sand-ili.The midgut lipase activity of the red pea aphid was (4.12 ±0.30)U/mL when they infested the cultivar Hunter river,but was only (1.63±0.10)U/mL (P <0.05)when they infested the variety Gannong No.5.Li-pase activity was (4.00 ±0.29 )U/mL when aphids had fed on the variety Weinan,and was (1.08 ±0.13 ) U/mL when feeding on the cultivar Gannong No.5.The midgut amylase activities of the red and green pea a-phids were (2.30 ±0.40)U/mL and (2.14 ±0.29 )U/mL,respectively when they had fed on the cultivar Hunter river.In summary,for pea aphid infestation of 8 alfalfa varieties with differing aphid resistance,a high negative linear correlation was observed between the resistance of the different alfalfa varieties and the prote-ase,lipase and amylase activities of the pea aphid,i.e.when the alfalfa aphid resistance is higher,the enzyme activity is lower.Hence it is concluded that the resistant alfalfa varieties restrict the midgut digestive enzyme activity of the pea aphid.关键词
豌豆蚜/苜蓿/蛋白酶/脂肪酶/淀粉酶/抗性Key words
pea aphid (Acyrthosiphon pisum )/alfalfa (Medicago sativa )/protease/lipase/amylase/resist-ance引用本文复制引用
达丽婷,朱亚灵,宋丽雯,胡桂馨,王森山..不同苜蓿品种对豌豆蚜中肠消化酶活性的影响[J].草业科学,2015,(10):80-87,8.基金项目
草业生态系统教育部重点实验室开放基金科研项目（CYZS-2011015）资助。 （）
