草业科学Issue(10)：88-98,11.DOI:10.11686/cyxb2014466
紫花苜蓿根腐病原菌--拟枝孢镰刀菌的鉴定及其生物学特性研究
Identification and biological characteristics of Fusarium sporotrichioide isolated from Medicago sativa root
摘要
Abstract
The strain BYE27-2-5 isolated from alfalfa (Medicago sativa )with root rot in north China was com-prehensively evaluated by analyses of its biological characteristics,morphology,and ITS sequence.The opti-mum temperatures for mycelium growth,sporulation,and spore germination were 25℃,30℃,and 28℃,re-spectively.The strain was able to grow in a pH range of 5.0 -11.0 and the optimal pH for sporulation and spore germination were 8.0 and 7.0,respectively.Alternative light conditions were optimal for mycelial growth whereas complete light conditions were optimal for sporulation and spore germination.Spores germina-ted most rapidly at 95% relative humidity or in water-drop conditions,and failed germinate at relative humidity levels below 75%.Dextrose peptone medium was the best medium for mycelial growth and spore germination and potato dextrose agar was the best medium for sporulation.The best carbon source for mycelial growth and spore germination was glucose and that for sporulation was sucrose.The best nitrogen source for mycelium growth was peptone and that for spore germination and sporulation was yeast extract.The lethal temperature for mycelia was 54℃ (10 min)and that for spores was 48℃ (10 min).Based on these biological characteristics and the results of an ITS sequence analysis,the isolate was identified as Fusarium sporotrichioide .关键词
紫花苜蓿/拟枝孢镰刀菌/病原鉴定/生物学特性Key words
alfalfa/Fusarium sporotrichioide/pathogen identification/biological characteristics引用本文复制引用
潘龙其,张丽,杨成德,袁庆华,王瑜,苗丽宏..紫花苜蓿根腐病原菌--拟枝孢镰刀菌的鉴定及其生物学特性研究[J].草业科学,2015,(10):88-98,11.基金项目
公益性行业（农业）科研专项项目（201303057）和国家科技支撑项目“优质多抗牧草新品种选育与良种繁育关键技术研究与示范”（2011BAN17B01）资助。 （）
