重庆医学Issue(21)：2500-2502,2510,4.DOI:10.3969/j.issn.1671-8348.2013.21.022
缺血预适应对大鼠肢体缺血再灌注后小肠损伤的保护效应及NO的作用
Protective effect of ischemia preconditioning and the role of nitric oxide on the small intestines injury following hind limbs ischemia reperfusion in rat *
摘要
Abstract
Objective To observe the protection effect of ischemic preconditioning to small intestines injury after limbs ischemia reperfusion ,and investigate the role of nitrogen monoxidum in the process .Methods 52 male Wistar rats were randomly divided in-to Sham group ,limbs ischemia-reperfusion (IR) group ,IPC + IR group and Nω-nitro-L-arginine methylester (L-NAME) group . The value of diamine oxidase (DAO) and nitrogen monoxidum (NO) in plasma and gut tissue ,the content of fluorescein isothiocya-nate lipopolysaccharide (FITC-LPS) in plasma ,and the value of cyclic guanosine monophosphate (cGMP) and malondialehyde (MDA) in gut tissue were measured ,respectively .The expression of P-selection and Caspase-3 were measured by immunohisto-chemistry .The apoptosis of intestinal cells was detected by terminal deoxynucleotidyl transferase -mediated dUTP nick end labe-ling (TUNEL) and the results were analysed quantitatively by automatic image analytical system .Results Compared with the LIR group ,the values of DAO and FITC-LPS in plasma decreased obviously in the IPC group ,and the level of NO in plasma increased obviously (P<0 .01) .The values of DAO ,NO and cGMP in gut tissue increased obviously ,the level of MDA and the expression of Caspase-3 and the apoptosis index (AI) were significantly decreased in IPC group (P<0 .01) .Compared with the IPC group ,the value of DAO and FITC-LPS were higher and the level of NO was lower in plasma of the L-NAME group (P<0 .01) .The values of DAO ,NO and cGM P in gut tissue obviously decreased ,the level of MDA significantly increased ,and the expression of Caspase-3 and the AI significantly upregulated in L-NAME group than those in IPC group (P<0 .01) .Conclusion IPC can improve the small intestine injury and the apoptosis following hind limbs ischemia reperfusion which may correlated with the increase in the level of NO .关键词
再灌注损伤/一氧化氮/细胞凋亡/缺血预适应/环磷酸鸟苷Key words
reperfusion injury/nitric oxide/cyclic guanosine monophosphate/apoptosis/ischemic preconditioning引用本文复制引用
刘燕,彭海兵,张博,周洪霞,杨秀红,张连元..缺血预适应对大鼠肢体缺血再灌注后小肠损伤的保护效应及NO的作用[J].重庆医学,2013,(21):2500-2502,2510,4.基金项目
河北省科技厅计划项目（12277793）；唐山市科技局科研基金资助课题（12140209A-27）。 （）
