重庆医学Issue(25)：3477-3480,4.DOI:10.3969/j.issn.1671-8348.2015.25.007
IL-22重组慢病毒促进肝细胞再生的作用研究
The effect of IL-22 recombinant lentivirus on proliferation of liver cell
摘要
Abstract
Objective To investigate the effect of IL-22 recombinant lentivirus on proliferation of liver cell.Methods We cultivated human normal hepatocyte L02 in vitro,IL-22 recombinant lentivirus infected L02,the efficiency of infection was detected after infection for 1,3,5 days by fluorescence microscop,and the expression of IL-22 was assayed by ELISA.Then set 3 treatment groups,including infected IL-22 recombinant lentivirus group,infected empty lentivirus group,uninfected group.After infection for 48 h,the expression of haptoglobin mRNA in L02 was assayed by RT-PCR.The proliferative activity of L02 was detected by MTT after infection for 48,72,96 h,respectively.Using immunocytochemistry tested the expression of proliferating cell nuclear antigen (PCNA),after treatment for 24 h.Results The efficiency of infection was up to(90.12±3.45)% after IL-22 recombinant lentivir-us infection for 3 days,it was highest,compared with infection for 1 day and 5days.According to our data,after infection for 3 days, the expressions of IL-22 was outstanding increase compared with infection for 1 day and 5 days(P <0.05).Haptoglobin mRNA ex-pressed significantly higher in infected IL-22 recombinant lentivirus group tha Ⅱ the other groups(P <0.01),but expressive differ-ence between infected empty lentivirus group and uninfected group had no statistical significance(P >0.05).The proliferative activi-ty of L02 was obviously more increased in infected IL-22 recombinant lentivirus group than that in infected empty lentivirus group and uninfected group each point in time(P <0.05).And the expression of PCNA was apparently higher in infected IL-22 recombi-nant lentivirus group than that in infected empty lentivirus group and uninfected group(P <0.01).Meanwhile,expressive difference between infected empty lentivirus group and uninfected group had no statistical significance(P >0.05 ).Conclusion IL-22 could promote the expression of haptoglobin mRNA and PCNA in L02,and enhance the proliferative activity of L02.Therefore,IL-22 may stimulate liver cell regeneration.关键词
白细胞介素-22/结合珠蛋白/增殖核抗原Key words
interleukin-22/haptoglobin/proliferating cell nuclear antigen分类
医药卫生引用本文复制引用
张红宾,罗红春,曾爱中,辛小娟..IL-22重组慢病毒促进肝细胞再生的作用研究[J].重庆医学,2015,(25):3477-3480,4.基金项目
重庆市科委基金项目(cstc2013jcyjA10078、cstc2012jjA10100) （cstc2013jcyjA10078、cstc2012jjA10100）
重庆市卫生局医学科研项目(2012-02-22)。 （2012-02-22）
