重庆医学Issue(26)：3605-3608,4.DOI:10.3969/j.issn.1671-8348.2015.26.002
IWR-1-endo 对三阴性乳腺癌抑制作用的研究
Study on inhibitory effect of IWR-1-endo to triple negative breast cancer
摘要
Abstract
Objective To investigate whether IWR-1-endo,a newly discovered small molecule inhibitor of Wnt signaling pathway,can suppress the proliferation of triple negative breast cancer cells MDA-MB-231 and nude mice tumor-bearing model and its relative potential mechanism.Methods MDA-MB-231 cells were treated by different concentrations of IWR-1-endo,and the cel-lular proliferation inhibition rate was calculated.The nude mice tumor-bearing model was constructed by intraperitoneal injection of IWR-1-endo (0.1 mL/10 g,once per 2 d).The long and short diameters of tumor were measured once every 4 d,in order to calcu-late the tumor volume.Mice were sacrificed after 4 weeks to collect tumor tissue for measuring and calculating the suppression rate of volume and weight.The tumor tissue was made into the paraffin sections for conducting Ki-67 staining and TUNEL staining.The proliferative index and apoptotic index were calculated.The expression of Axin 1,Axin 2,β-catenin and p-β-catenin protein was de-tected by Western blot.Results IWR-1-endo could obviously inhibit the growth of MDA-MB-231 cells in a concentration-dependent manor (IC50 value was 180 nmol/ L).The tumor volume suppression rate of IWR-1-endo was 42.2% and its tumor weight sup-pression rate was 46.6%.Compared with the control group,the apoptotic index in the IWR-1-endo group was significantly in-creased,the difference was statistically significant,while the difference of proliferative index had no statistical significance.The Western blot results showed that the expression amount of Axin 1 and Axin 2 in the cells and tissues was significantly increased, theβ-catenin expression was obviously decreased,while theβ-catenin level was reduced and the p-β-catenin level was increased.Con-clusion IWR-1-endo can inhibit the Wnt signal pathway by stabilizing Axin,and realize the inhibition on TNBC cell line and growth of nude mice tumor-bearing model.关键词
乳腺肿瘤/Wnt 蛋白质类/IWR-1-endoKey words
breast neoplasms/wnt proteins/IWR-1-endo分类
医药卫生引用本文复制引用
江燕,曹译心,张翠薇,张旭..IWR-1-endo 对三阴性乳腺癌抑制作用的研究[J].重庆医学,2015,(26):3605-3608,4.基金项目
四川省卫生厅项目(80173) （80173）
泸州医学院项目(2010108)。 （2010108）
