重庆医学Issue(29)：4066-4069,4.DOI:10.3969/j.issn.1671-8348.2015.29.013
脂多糖对气道上皮细胞分泌炎症因子的影响
The inflation of lipopolysaccharide to cytokine in airway epithelial cell
摘要
Abstract
Objective To detect different cytokines (TGF‐β,MCP‐1 ,TNF‐α,ICAM‐1 ,ICAM‐2) concentration after lipopo‐lysaccharides(LPS) stimulation airway epithelial cells at the same time ,to explore the role of LPS in the airway epithelial cells se‐creting a variety of cytokines .Methods A549 cell strain was cultivated and interfered by different concentrations LPS(0 ,0 .1 ,1 .0 , 10 .0 ,20 .0 ,30 .0 ,50 .0 ,100)μg/mL after 2 ,4 ,8 ,24 ,30 h .Collected culture medium and total protein in the cell ,detected volume of cytokines TGF‐β,MCP‐1 ,TNF‐α,ICAM‐1 and ICAM‐2 secreted by A549 cell using enzyme‐linked immunosorbent assays (ELISA) ,to analysed the changes of above‐mentioned cytokines at the different time period .Results First ,LPS interfered the air‐way epithelial cells secreting TGF‐β,MCP‐1 ,TNF‐α,ICAM‐1 and ICAM‐2 ;the secretion of ICAM‐1 peaked when the concentration of LPS was 20 .0μg/mL and the time was 4 h and 8 h;the secretion of MCP‐1 peaked when the concentration of LPS was 50 .0μg/mL and the time was 4 h .Second ,the volume of cytokines TGF‐β,MCP‐1 ,TNF‐α,ICAM‐1 ,ICAM‐2 was related to the concentra‐tion and the period of LPS .Compared with the control group ,the maximum secretion of TNF‐α,MCP‐1 and ICAM‐1 were increased at all the different time ,the difference was statistically significant (P<0 .01);whereas at the 30 h ,the secretion of TGF‐βwas de‐creased and the secretion of ICAM‐2 was not different from the control group(P> 0 .05) ,compared with the control group ,the maximum secretion of TGF‐βand ICAM‐2 were increased at all the other different time ,the difference was statistically significant (P<0 .01) .Conclusion LPS influenced the volume of cytokines TGF‐β,MCP‐1 ,TNF‐α,ICAM‐1 and ICAM‐2 are related to the time and concentration of LPS ,the particular intervention time and concentration enabled them reaches maximum .关键词
脂多糖类/A549细胞/炎症因子Key words
lipopolysaccharides/A549 cell/cytokines分类
医药卫生引用本文复制引用
舒艳,蔡颖,况九龙..脂多糖对气道上皮细胞分泌炎症因子的影响[J].重庆医学,2015,(29):4066-4069,4.基金项目
国家自然科学基金资助项目（81060011／H0118）。 （）
