重庆医学Issue(32)：4483-4486,4.DOI:10.3969/j.issn.1671-8348.2015.32.007
心肺复苏术后大鼠海马组织高迁移率族蛋白B1和NF-κB的表达及相关性分析
Time-course of HMGB1 and NF-κB expression and its implication in the hippocampus of rats after cardiopulmonary resuscitation
摘要
Abstract
Objective To observe the changes of high mobility group box 1(HMGB1) and nuclear factorκB(NF‐κB) expres‐sion in the hippocampus of rats after cardiopulmonary resuscitation so as to unravel the role of HMGB 1 and NF‐κB in neuroin flam‐mation .Methods Totally 40 Sprague‐Dawley rats were randomly divided into shame‐operated group and recover group [including 2 ,6 ,12 ,24 and 48 h of 5sub‐groups after restoration of spontaneous circulation (ROSC)] .The animals were sacrificed and hippo‐campus were removed at the indicated time .Pathological changes were observed at each time point .The expression of HMGB1 and NF‐κB were determined using RT‐PCR and Western blot respectively .Results There were no histopathological in the hippocampus of rats in shame‐operated group ,brain tissue appeared change of ischemia pathology in recover group ,it was the most severest at ROSC 24 h and still obviously at ROSC 48 h time point .HMGB1 mRNA and NF‐κB mRNA expression in the hippocampus of rats of recover group increased obviously along with the prolongation of time following ROSC and reached its peak at ROSC 24 h(P<0 . 01) ,much higher than that of shame‐operated group ;the HMGB1 level in the hippocampus of rats after recover significantly de‐clined at 2 h after ROSC and increased obviously at 6 ,12 h and reached peak 24 h later ,then decreased 48 h later(P<0 .01) ,there was positive correlation between the expression of HMGB1 and NF‐κB protein .Conclusion HMGB1/NF‐κB signaling pathway may play an important role in the early stages of brain injury after cardiopulmonary resuscitation .Targeted therapies of this path way would be possible to open a new avenue for preventing neuroinflammation after recover .关键词
心肺复苏术/NF-κB/高迁移率族蛋白B1/神经炎症损伤Key words
cardiopulmonary resuscitation/NF-κB/high mobility group box 1/neuroinflammation分类
医药卫生引用本文复制引用
侯安然,康秀文,陈晓兵,王言理,刘克喜..心肺复苏术后大鼠海马组织高迁移率族蛋白B1和NF-κB的表达及相关性分析[J].重庆医学,2015,(32):4483-4486,4.基金项目
江苏省连云港市科技局科研基金资助项目（sh1001）。 （）
