重庆医学Issue(32)：4490-4492,3.DOI:10.3969/j.issn.1671-8348.2015.32.009
石菖蒲大孔树脂不同洗脱部位高效液相图谱与抗抑郁效应的关联性研究
Correlation study on high performance liquid chromatography and antidepressant effect of different eluting fractions of acorus tatarinowii schott macroporous resin
摘要
Abstract
Objective To research the correlation on high performance liquid chromatography (HPLC) and antidepressant effect of different elution fractions of acorus tatarinowii schott macroporous resin .Methods Prepared different fractions by using AB‐8 macroporous resin .Establish HPLC characteristic chromatograms of crude drugs and different elution fractions from AB‐8 acorus tatarinowii schott macroporous resin by using gradient elusion .Confirm characteristic peaks in chromatograms .despair mod‐els of male mice ,including tails suspension test (TST ) ,forced swimming test (FST ) were used to evaluate antidepressant effects by taking orally .Results HPLC chromatograms of crude drugs and different fraction of acorus tatarinowii schott showed that they had same characteristic peaks .Elution of 90% and 70% alcohol solution from AB‐8 macroporous resin can obviously reduce TST and FST time .The anti‐depressant effect were associated with characteristic peaks on chromatogram .Conclusion Acorus tatarinowii schott has anti‐depressant effect ,and mainly from 70% and 90% alcohol solution eluted from AB‐8 macroporous resin .HPLC char‐acteristic chromatography and data relationship between relative peaks can be used to judge the antidepressant effects of acorus ta‐tarinowii schott ,and give reference to research and product development .关键词
石菖蒲/AB-8大孔树脂不同洗脱部位/高效液相图谱/抗抑郁Key words
acorus tatarinowii schott/different fraction eluted from AB-8 macroporous resin/high performance liquid chro-matography/anti-depression分类
医药卫生引用本文复制引用
周雅菲,朱显婷,邓雨晴,周威..石菖蒲大孔树脂不同洗脱部位高效液相图谱与抗抑郁效应的关联性研究[J].重庆医学,2015,(32):4490-4492,3.基金项目
湖北省教育厅科学技术研究计划重点项目（D20131702）；湖北省卫生厅科研项目（JX6B100）。 （）
