重庆医学Issue(32)：4493-4495,4498,4.DOI:10.3969/j.issn.1671-8348.2015.32.010
人胰腺癌PANC-1细胞株中不同亚群放射敏感性的比较分析
Comparison analysis of radiosensitive in different subsets of pancreatic adenocarcinoma PANC-1 cell line
摘要
Abstract
Objective To investigate the radiosensitivity of stem cells in pancreatic adenocarcinoma PANC‐1 cell line and the possible mechanism of its radiation resistance .Methods Using flow cytometry ,the cells were isolated and sorted into CD44+CD24+ ,CD44-CD24+ ,CD44+CD24- ,and CD44-CD24- .Multi‐target click model was used to fit cell survival curves and deter‐mine the sensitizer enhancement ratio .The apoptosis and cycle distribution of the four cell subsets were determined using flow cy‐tometry ,and the level of ROS was determined by DCFH‐DA probe .Results The ratio of CD44+ and CD24+ in the sorted PANC‐1 cell line was 92 .0% and 4 .7% respectively .Before radiation ,there was no statistically significant difference between four groups (P>0 .05);After treated with 6MV‐X ray ,The ratio of apoptosis was the lowest in CD44+CD24+ (P<0 .01);The percentage G0/G1 cell was the highest in CD44+CD24+ (P<0 .01) ,the sensitizer enhancement ratio of CD44+ CD24- ,CD44-CD24+ and CD44-CD24- were 1 .61 ,1 .81 ,1 .94 ,respectively .The level of ROS in CD44+CD24+ was lower (P<0 .01) .Conclusion Tumor stem cells of pancreatic adenocarcinoma have properties of a lower level of ROS and relative stationary that maybe the reasons of radio resist‐ance .关键词
胰腺肿瘤/PANC-1/干性细胞/放射敏感性Key words
pancreatic neoplasms/PANC-1/stem cell/radiosensitive分类
医药卫生引用本文复制引用
王磊,胡晨曦,夏铀铀,宋大安,黎世秋,蒋晓东..人胰腺癌PANC-1细胞株中不同亚群放射敏感性的比较分析[J].重庆医学,2015,(32):4493-4495,4498,4.基金项目
江苏省连云港市科技局项目基金（SH1218）。 （）
