重庆医学Issue(32)：4553-4555,4559,4.DOI:10.3969/j.issn.1671-8348.2015.32.031
Ⅱ型胶原酶消化时间对兔软骨细胞生物学特性的影响
The impact of collagenaseⅡdigestion time on the biological characteristics of rabbit chondrocytes
摘要
Abstract
Objective To investigate the best digestion time for rabbit rib cartilage cells separation by collagenase Ⅱ diges‐tion method .Methods Rabbit cartilage tissue was taken from 3‐month‐old New Zealand white rabbits ,and then digested in 0 .2%collagenase Ⅱ solution for 4 ,8 ,12 ,16 and 20 h under vibration at 37 ℃ ,respectively .The corresponding experiments were named as group A ,B ,C ,D ,and E .The obtained primary cells were cultured and passaged after counting .The cell morphology ,proliferation rate ,secretion of extracellular matrix of passage 2 cells were compared to chose the best digestion time .Results The primary chon‐drocytes obtained in group C ,D and E were more than the other two group A and B .The chondrocytes cytoactive in group C and D were better than the other three group A ,B and E .The passage 2 chondrocytes in group C ,D had better secretion of extracellular matrix capability than the other three groups .Conclusion The best collagenase Ⅱ digestion time is 12 to 16 h ,at this digestion time period ,more primary chondrocytes with high cytoactive and strong proliferation capability can be obtained ,and the phenotype of their passage 2 cells is quite good .关键词
胶原酶类/软骨细胞/生物学特性/细胞培养技术Key words
collagenase/chondrocytes/biological characteristics/cell culture techniques分类
医药卫生引用本文复制引用
袁德超,陈竹,邓尚,冯刚..Ⅱ型胶原酶消化时间对兔软骨细胞生物学特性的影响[J].重庆医学,2015,(32):4553-4555,4559,4.基金项目
国家自然科学基金资助项目（81171472、81201407）；四川省教育厅创新团队资助项目（13 T D0030）；南充市科技局科研项目（14A0073）。 （）
